Titre du document / Document title

Mutated-leptin gene transfer induces increases in body weight by electroporation and hydrodynamics-based gene delivery in mice

Auteur(s) / Author(s)

LAN XIANG ; MURAI Atsushi ; MURAMATSU Tatsuo ;

Résumé / Abstract

To investigate whether in vivo gene transfer causes leptin-antagonistic effects on food intake, animal body weight and fat tissue weight, the R128Q mutated-leptin gene, an R to Q substitution at position 128 of mouse leptin, was transferred into mouse liver and leg muscle by electroporation and hydrodynamics-based gene delivery. Mutated-leptin gene transfer by electroporation caused significant increases in body weight at 5 days and after (5.4% increase relative to control; p<0.05). Hydrodynamics-based gene delivery of the mutated-leptin gene also caused an increase in body weight (3.0% increase relative to control: p<0.05). Mutated-leptin gene transfer by electroporation significantly increased the tissue weight of epididymal white fat and neuropeptide Y mRNA expression in the hypothalamus compared with those of the control group 3 weeks after gene transfer (p<0.05). These results suggest that mutated-leptin gene transfer successfully produced leptin-antagonistic effects by modulating the central regulator of energy homeostasis. Also, the extent of leptin-antagonistic effects by electroporation was much higher than hydrodynamics-based gene delivery, with at least single gene transfer.

Revue / Journal Title

International journal of molecular medicine   ISSN 1107-3756 

Source / Source

2005, vol. 16, n^o6, pp. 1015-1020 [6 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

D.A. Spandidos, Athens, GRECE  (1998) (Revue)

Mots-clés d'auteur / Author Keywords

leptin ; mutated leptin ; electroporation ; hydrodynamics- based gene delivery ; body weight ; food intake ;

Localisation / Location

INIST-CNRS, Cote INIST : 27038, 35400013526646.0070