Titre du document / Document title
Transgene transmission to progeny by oMt1a-oGH transgenic mice
Auteur(s) / Author(s)
THOMAS A. D. (1) ;
MURRAY J. D. (1) ;
OBERBAUER A. M. (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Animal Science, University of California, Davis, One Shields Ave, Davis CA 95616, ETATS-UNIS
Résumé / Abstract
Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation of a founder animal, but decreases in subsequent generations. In the present study, the ovine metallothionein la-ovine growth hormone (oMtla-oGH) transgenic mouse was used to determine whether transgene transmission rate to progeny was affected by overexpression of ovine growth hormone in the transgenic parent. The oMtla-oGH mouse is a useful model for assessing transgene transmission, as the construct is easily regulatable and transgene inactivation results in a return of plasma GH to basal levels. Male and female hemizygous oMtla-oGH mice were assigned to 1 of 3 treatment groups: (1) mice never actively expressing the transgene, (2) mice actively expressing the transgene from 3 weeks of age, and (3) mice actively expressing the transgene from 3 to 11 (males) or 3 to 8 (females) weeks of age. Transgenic mice were mated to wild type animals and the resulting progeny were genotyped. Males never actively expressing the transgene passed on the transgene to progeny in a Mendelian fashion, while males actively expressing the transgene transmitted the transgene to a smaller than expected number of progeny. However, following inactivation of the oMtla-oGH construct in transgenic males, subsequent offspring demonstrated Mendelian inheritance of the transgene. In contrast, females expressing the transgene from 3 to 8 weeks of age were able to pass on the oMtla-oGH construct in a Mendelian fashion, but females from other treatment groups were not. In oMtla-oGH males, reduced transgene transmission appears to be due to selection against transgenic gametes. In females, however, selection against the transgenic genotype likely occurs at the embryonic level.
Revue / Journal Title
Transgenic research
ISSN
0962-8819
Source / Source
2005, vol. 14, n
o4, pp. 441-448 [8 page(s) (article)] (1 p.1/4)
Langue / Language
Anglais
Editeur / Publisher
Springer, Dordrecht, PAYS-BAS
(1991)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 26051, 35400013158218.0090
Nº notice refdoc (ud4) : 17065121
