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Titre du document / Document title

Transgene transmission to progeny by oMt1a-oGH transgenic mice

Auteur(s) / Author(s)

THOMAS A. D. (1) ; MURRAY J. D. (1) ; OBERBAUER A. M. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Animal Science, University of California, Davis, One Shields Ave, Davis CA 95616, ETATS-UNIS

Résumé / Abstract

Most studies utilizing transgenic technology focus on the impact to traits of interest, rather than propagation of the transgene to offspring. In animals containing growth hormone constructs, transgene transmission to progeny follows a Mendelian pattern of inheritance in the first few generations following generation of a founder animal, but decreases in subsequent generations. In the present study, the ovine metallothionein la-ovine growth hormone (oMtla-oGH) transgenic mouse was used to determine whether transgene transmission rate to progeny was affected by overexpression of ovine growth hormone in the transgenic parent. The oMtla-oGH mouse is a useful model for assessing transgene transmission, as the construct is easily regulatable and transgene inactivation results in a return of plasma GH to basal levels. Male and female hemizygous oMtla-oGH mice were assigned to 1 of 3 treatment groups: (1) mice never actively expressing the transgene, (2) mice actively expressing the transgene from 3 weeks of age, and (3) mice actively expressing the transgene from 3 to 11 (males) or 3 to 8 (females) weeks of age. Transgenic mice were mated to wild type animals and the resulting progeny were genotyped. Males never actively expressing the transgene passed on the transgene to progeny in a Mendelian fashion, while males actively expressing the transgene transmitted the transgene to a smaller than expected number of progeny. However, following inactivation of the oMtla-oGH construct in transgenic males, subsequent offspring demonstrated Mendelian inheritance of the transgene. In contrast, females expressing the transgene from 3 to 8 weeks of age were able to pass on the oMtla-oGH construct in a Mendelian fashion, but females from other treatment groups were not. In oMtla-oGH males, reduced transgene transmission appears to be due to selection against transgenic gametes. In females, however, selection against the transgenic genotype likely occurs at the embryonic level.

Revue / Journal Title

Transgenic research    ISSN  0962-8819 

Source / Source

2005, vol. 14, no4, pp. 441-448 [8 page(s) (article)] (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1991) (Revue)

Mots-clés anglais / English Keywords

Vertebrata

;

Mammalia

;

Rodentia

;

Somatotropin hormone

;

Mouse

;

Transgenic animal

;

Transmission

;

Mots-clés français / French Keywords

Vertebrata

;

Mammalia

;

Rodentia

;

STH

;

Souris

;

Animal transgénique

;

Transmission

;

Mots-clés espagnols / Spanish Keywords

Vertebrata

;

Mammalia

;

Rodentia

;

STH

;

Ratón

;

Animal transgénico

;

Transmisión

;

Mots-clés d'auteur / Author Keywords

growth hormone

;

non-Mendelian inheritance

;

oMtla-oGH transgenic mice

;

transgene transmission

;

Localisation / Location

INIST-CNRS, Cote INIST : 26051, 35400013158218.0090

Nº notice refdoc (ud4) : 17065121



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