Titre du document / Document title

Cannabinoids selectively inhibit proliferation and induce death of cultured human glioblastoma multiforme cells

Auteur(s) / Author(s)

MCALLISTER Sean D. ⁽¹⁾ ; CHAN Calvin ⁽¹⁾ ; TAFT Ryan J. ⁽¹⁾ ; LUU Tri ⁽¹⁾ ; ABOOD Mary E. ⁽¹⁾ ; MOORE Dan H. ^{(1 2)} ; ALDAPE Ken ⁽³⁾ ; YOUNT Garret ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ California Pacific Medical Center Research Institute, San Francisco, CA, ETATS-UNIS
⁽²⁾ Department of Epidemiology and Biostatistics, University of California San Francisco, San Francisco, CA, ETATS-UNIS
⁽³⁾ Department of Pathology, University of Texas MD Anderson Cancer Center, Houston, TX, ETATS-UNIS

Résumé / Abstract

Normal tissue toxicity limits the efficacy of current treatment modalities for glioblastoma multiforme (GBM). We evaluated the influence of cannabinoids on cell proliferation, death, and morphology of human GBM cell lines and in primary human glial cultures, the normal cells from which GBM tumors arise. The influence of a plant derived cannabinoid agonist, Δ⁹-tetrahydrocannabinol (Δ⁹-THC), and a potent synthetic cannabinoid agonist, WIN 55,212-2, were compared using time lapse microscopy. We discovered that Δ⁹-THC decreases cell proliferation and increases cell death of human GBM cells more rapidly than WIN 55,212-2. Δ⁹-THC was also more potent at inhibiting the proliferation of GBM cells compared to WIN 55,212-2. The effects of Δ⁹-THC and WIN 55,212-2 on the GBM cells were partially the result of cannabinoid receptor activation. The same concentration of Δ⁹-THC that significantly inhibits proliferation and increases death of human GBM cells has no significant impact on human primary glial cultures. Evidence of selective efficacy with WIN 55,212-2 was also observed but the selectivity was less profound, and the synthetic agonist produced a greater disruption of normal cell morphology compared to Δ⁹-THC.

Revue / Journal Title

Journal of neuro-oncology   ISSN 0167-594X   CODEN JNODD2 

Source / Source

2005, vol. 74, n^o1, pp. 31-40 [10 page(s) (article)] (33 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1983) (Revue)

Mots-clés anglais / English Keywords

Malignant tumor ; Central nervous system disease ; Malignant glioma ; Enzyme ; Microscopy ; Extracellular signal-regulated protein kinase ; Human ; Cell culture ; Cannabinoid ; Glioblastoma multiforme ; Nervous system diseases ;

Mots-clés français / French Keywords

Tumeur maligne ; Système nerveux central pathologie ; Gliome malin ; Enzyme ; Microscopie ; Extracellular signal-regulated protein kinase ; Homme ; Culture cellulaire ; Cannabinoïde ; Glioblastome multiforme ; Système nerveux pathologie ;

Mots-clés espagnols / Spanish Keywords

Tumor maligno ; Sistema nervosio central patología ; Glioma maligno ; Enzima ; Microscopía ; Extracellular signal-regulated protein kinase ; Hombre ; Cultivo celular ; Canabinoide ; Glioblastoma multiforme ; Sistema nervioso patología ;

Mots-clés d'auteur / Author Keywords

cannabinoid ; delta-9-tetrahydrocannabinol ; extracellular signal regulated kinases ; glioblastma multiforme ; time lapse microscopy ;

Localisation / Location

INIST-CNRS, Cote INIST : 20812, 35400013237715.0040