Titre du document / Document title

Heat immunotherapy using magnetic nanoparticles and dendritic cells for T-lymphoma

Auteur(s) / Author(s)

TANAKA Kouji ⁽¹⁾ ; ITO Akira ⁽¹⁾ ; KOBAYASHI Takeshi ⁽¹⁾ ; KAWAMURA Tatsuyoshi ⁽²⁾ ; SHIMADA Shinji ⁽²⁾ ; MATSUMOTO Kazuhiko ⁽³⁾ ; SAIDA Toshiaki ⁽³⁾ ; HONDA Hiroyuki ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Biotechnology, School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, JAPON
⁽²⁾ Department of Dermatology, School of Medicine, Yamanashi University, 1110 Shimogato, Tamah, Nakakoma, Yamanashi 409-3898, JAPON
⁽³⁾ Department of Dermatology, School of Medicine, Shinshu University, Asahi, Matsumoto, Nagano 390-8621, JAPON

Résumé / Abstract

Dendritic cells (DCs) are potent antigen-presenting cells that play important roles in regulating immune responses in cancer. Immunotherapy using these immunocytes has become an accepted therapeutic modality. We previously reported that hyperthermia using magnetic nanoparticles induces antitumor immunity, which could be activated by adjuvant including cytokines. In the present study, we investigated the therapeutic effects of hyperthermia combined with DC immunotherapy on mouse EL4 T-lymphoma. Magnetite cationic liposomes (MCLs) have a positive surface charge and generate heat in an alternating magnetic field (AMF) due to hysteresis loss. MCLs were injected into an EL4 nodule in C57BL/6 mice, which were subjected to AMF for 30 min. The temperature at the surface of the tumor reached 45°C and was maintained by controlling the magnetic field intensity. Hyperthermia treatment was repeated twice with 24 h intervals. After hyperthermia, immature DCs were directly injected into the EL4 nodule. As a result, complete regression of tumors in 75% (6/8) of the mice was observed, while the percentage of complete regression of tumors was 12.5% (1/8) in the case of mice treated by hyperthermia alone. This novel cancer therapy, which we have termed heat immunotherapy, may be applicable to patients with advanced malignancies.

Revue / Journal Title

Journal of bioscience and bioengineering   ISSN 1389-1723 

Source / Source

2005, vol. 100, n^o 1 (134 p.)  [Document : 4 p.] (27 ref.), pp. 112-115 [4 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier Science, Amsterdarm, PAYS-BAS  (1999) (Revue)

Mots-clés anglais / English Keywords

Liposome ; Magnetite ; Hyperthermia ; Therapy ; Malignant tumor ; Tumor cell ; Dendritic cell ; Nanoparticle ; Magnetic ; Immunotherapy ; Heat ;

Mots-clés français / French Keywords

Liposome ; Magnétite ; Hyperthermie ; Thérapie ; Tumeur maligne ; Cellule tumorale ; Cellule dendritique ; Nanoparticule ; Magnétique ; Immunothérapie ; Chaleur ;

Mots-clés espagnols / Spanish Keywords

Liposoma ; Magnetita ; Hipertermia ; Terapia ; Tumor maligno ; Célula tumoral ; Célula dendrítica ; Nanopartícula ; Magnético ; Inmunoterapia ; Calor ;

Mots-clés d'auteur / Author Keywords

cancer therapy ; dendritic cell ; immunotherapy ; hyperthermia ; magnetite cationic liposome ;

Localisation / Location

INIST-CNRS, Cote INIST : 8234, 35400013836441.0160