Titre du document / Document title

Effects of heavy water (D₂O) on human pancreatic tumor cells

Auteur(s) / Author(s)

HARTMANN Johannes ⁽¹⁾ ; BADER Yvonne ⁽¹⁾ ; HORVATH Zsuzsanna ⁽¹⁾ ; SAIKO Philipp ⁽¹⁾ ; GRUSCH Michael ⁽¹⁾ ; ILLMER Christoph ⁽¹⁾ ; MADLENER Sibylle ⁽¹⁾ ; FRITZER-SZEKERES Monika ⁽¹⁾ ; HELLER Nicole ⁽²⁾ ; ALKEN Rudolf-Giesbert ⁽²⁾ ; SZEKERES Thomas ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Clinical Institute of Medical and Chemical Laboratory Diagnostics, Medical University of Vienna, General Hospital of Vienna, Waehringer Guertel 18-20, 1090 Vienna, AUTRICHE
⁽²⁾ BDD Berolina Drug Development GmbH, Fontanestrasse 84-89, 15366 Neuenhagen, ALLEMAGNE

Résumé / Abstract

Background: Pancreatic cancer constitutes an entity which is difficult to treat and, therefore, mostly fatal. Since heavy water (deuterium oxide, D₂O) was shown to be active in various cancer cell lines in vitro and in vivo, we now investigated its effects in human pancreatic tumor cells. Materials and Methods: The cytotoxic effects of D₂O were examined in three pancreatic cancer cell lines (AsPC-1, BxPC-3 and PANC-1). Induction of apoptosis was determined by Hoechst/propidium iodide double staining and cell cycle distribution was investigated by FACS analysis. Results: Employing a clonogenic assay, D₂O yielded IC₅₀ values of 15%, 18% and 27% in AsPC-1, PANC-1 and BxPC-3 cells, respectively, and led to the induction of apoptosis when compared to untreated controls. Moreover, D₂O caused a cell cycle arrest in the G2-M-phase (BxPC-3, PANC-1) or in the S-phase (AsPC-1). Conclusion: It is hoped that D₂O might offer an additional option for the treatment of pancreatic carcinomas.

Revue / Journal Title

Anticancer research   ISSN 0250-7005 

Source / Source

2005, vol. 25, n^o5, pp. 3407-3411 [5 page(s) (article)] (18 ref.)

Langue / Language

Anglais

Editeur / Publisher

International Institute of Anticancer Research, Attiki, GRECE  (1980) (Revue)

Mots-clés anglais / English Keywords

Malignant tumor ; Pancreatic disease ; Digestive diseases ; Pancreatic tumor ; Cancerology ; Distribution ; Cell cycle ; Cell death ; Apoptosis ; Tumor cell ; Human ; Water ; Pancreas cancer ;

Mots-clés français / French Keywords

Tumeur maligne ; Pancréas pathologie ; Appareil digestif pathologie ; Tumeur pancréas ; Cancérologie ; Distribution ; Cycle cellulaire ; Mort cellulaire ; Apoptose ; Cellule tumorale ; Homme ; Eau ; Cancer pancréas ;

Mots-clés espagnols / Spanish Keywords

Tumor maligno ; Páncreas patología ; Aparato digestivo patología ; Cancerología ; Distribución ; Ciclo celular ; Muerte celular ; Apoptosis ; Célula tumoral ; Hombre ; Agua ; Cáncer del páncreas ;

Mots-clés d'auteur / Author Keywords

Heavy water (deuterium oxide ; D2O) ; apoptosis ; cell cycle distribution ; pancreatic cancer ; AsPC-1 ; BxPC-3 and PANC-1 cells ;

Localisation / Location

INIST-CNRS, Cote INIST : 19426, 35400013148771.0350