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Titre du document / Document title

Characterization of a deletion allele of a sorghum Myb gene yellow seedl showing loss of 3-deoxyflavonoids

Auteur(s) / Author(s)

BODDU Jayanand (1) ; SVABEK Catherine (1) ; IBRAHEEM Farag (1) ; JONES A. Daniel (2) ; CHOPRA Surinder (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Crop & Soil Sciences, 252 Agricultural Sciences & Industries Building, The Pennsylvania State University, University Park, PA 16802, ETATS-UNIS
(2) Department of Chemistry, Pennsylvania State University, University Park, PA 16802, ETATS-UNIS

Résumé / Abstract

In sorghum, 3-deoxyflavonoid pigments or phlobaphenes observed in the pericarp of mature seed are derived from flavan-4-ols through the flavonoid biosynthetic pathway. We show here that phlobaphenes accumulation in pericarp, glumes and leaves is genetically linked with the functional yellow seed 1 (y1) gene. Molecular and genetic analysis was performed on a loss of function allele of y1 present in the line BTx623. This sorghum line does not accumulate any detectable levels of flavan-4-ols or visible phlobaphenes in pericarp, glumes and leaves. Molecular structure of the y1[BTx623] showed a deletion of 3218 bp which removes 5' non-coding, putative promoter, exon1, intron1, exon2, and part of the intron2 sequences. The null y1 allele designated as y1-ww (white pericarp, white glume) is not transcribed and this results in a loss of Y1-regulated expression of structural genes needed for the biosynthesis of flavan-4-ols. Further LC-MS analysis of seed extracts of a functional y1 allele detected the presence of positively charged compounds known as 3-deoxyanthocyanidins. Compounds identified were apigeninidin, luteolinidin, and a methoxylated derivative of apigeninidin. These compounds were not detected in BTx623 seed extracts. Previous studies have shown that 3-deoxyanthocyanidins are induced in sorghum leaves challenged with Colletotrichum sublineolwn, a fungus that causes anthracnose in sorghum. Our results now provide an evidence for a common flavonoid pathway that may lead to the biosynthesis of flavan-4-ols and 3-deoxyanthocyanidins in sorghum.

Revue / Journal Title

Plant science    ISSN  0168-9452   CODEN PLSCE4 

Source / Source

2005, vol. 169, no3, pp. 542-552 [11 page(s) (article)] (42 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Shannon, IRLANDE  (1985) (Revue)

Mots-clés anglais / English Keywords

Thallophyta

;

Fungi

;

Fungi Imperfecti

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Colletotrichum

;

Sorghum

;

Phytoalexin

;

Biosynthesis

;

Molecular structure

;

Plant leaf

;

Seeds

;

Gene

;

Mots-clés français / French Keywords

Thallophyta

;

Fungi

;

Fungi Imperfecti

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Colletotrichum

;

Sorghum

;

Phytoalexine

;

Biosynthèse

;

Structure moléculaire

;

Feuille végétal

;

Graine

;

Gène

;

Mots-clés espagnols / Spanish Keywords

Thallophyta

;

Fungi

;

Fungi Imperfecti

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Gramineae

;

Colletotrichum

;

Sorghum

;

Fitoalexina

;

Biosíntesis

;

Estructura molecular

;

Hoja vegetal

;

Semillas

;

Gen

;

Mots-clés d'auteur / Author Keywords

Anthracnose

;

Flavan-4-ols

;

MYB

;

Phytoalexins

;

Sorghum

;

3-Deoxyanthocyanidins

;

Localisation / Location

INIST-CNRS, Cote INIST : 15982, 35400013146510.0100

Nº notice refdoc (ud4) : 16983977



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