Titre du document / Document title

Use of polymyxin as an endotoxin blocker in the prevention of diffuse lamellar keratitis in an animal model

Auteur(s) / Author(s)

MORCK Douglas W. ⁽¹⁾ ; HOLLAND Simon P. ^{(2 3)} ; CERI Howard ⁽¹⁾ ; HANCOCK Robert ⁽⁴⁾ ; SCOTT-GOUGH Monisha ⁽⁴⁾ ; NUGYEN Van ⁽¹⁾ ; KEITH Erin J. ^{(2 3)} ; LEE Tracy L. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Life and Environmental Sciences, Animal Resource Centre, University of Calgary, Calgary, Alberta, CANADA
⁽²⁾ Department of Ophthalmology, University of British Columbia, CANADA
⁽³⁾ Pacific Laser Eye Centre, Vancouver, British Columbia, CANADA
⁽⁴⁾ Department of Microbiology and Immunology, University of British Columbia, CANADA

Résumé / Abstract

PURPOSE: To determine whether bacterial endotoxin, lipopolysaccharide (LPS), could induce diffuse lamellar keratitis (DLK) in an animal model and whether DLK could be prevented by endotoxin blockers such as polymyxin. METHODS: Laser in situ keratomileusis (LASIK) flaps were created in rabbit eyes. The stromal bed was treated with 20 pg of Burkhoideria cepacia LPS or balanced salt solution (BSS). Development of DLK. histological degree of inflammation, and presence of LPS detected by anti-LPS antibody were evaluated after 48 boors. In a second experiment, all eyes received LPS and were randomly assigned to receive either polymyxin in the form of two drops of Polytrim (Allergan, Irvine, Calif) on the stromal bed or two drops of BSS. RESULTS: In the animal model study, LPS was significantly associated with the development of DLK (P<.05, n=30). Infiltration with polymorphonuclear cells and presence of DLK were founcs in LPS treated eyes but not. in controls. In the second experiment, 4 (27%) of 15 eyes that received polymyxin in addition to LPS developed DLK compared to 18 (95%) of 19 eyes that received only LPS (P<.05, n=34). There was a trend towards higher flap displacement in polymysm treated eyes but this was not significant (P=.07). CONCLUSIONS: Diffuse lamellar keratitis in a rab bit model can be caused by bacterial endotoxin (LPS). Endotoxin biockers, such as polymyxin, are effective in decreasing the incidence of endotoxin-induced DLK in a rabbit model.

Revue / Journal Title

Journal of refractive surgery   ISSN 1081-597X   CODEN JRSUFZ 

Source / Source

2005, vol. 21, n^o2, pp. 152-157 [6 page(s) (article)] (14 ref.)

Langue / Language

Anglais

Editeur / Publisher

Slack, Thorofare, NJ, ETATS-UNIS  (1995) (Revue)

Mots-clés anglais / English Keywords

Anterior segment disease ; Eye disease ; Keratopathy ; Toxin ; Ophthalmology ; Surgery ; Animal model ; Prevention ; Endotoxin ; Use ; Diffuse lamellar keratitis ;

Mots-clés français / French Keywords

Segment antérieur pathologie ; Oeil pathologie ; Kératopathie ; Toxine ; Ophtalmologie ; Chirurgie ; Modèle animal ; Prévention ; Endotoxine ; Utilisation ; Kératite lamellaire diffuse ;

Mots-clés espagnols / Spanish Keywords

Segmento anterior patología ; Ojo patología ; Queratopatía ; Toxina ; Oftalmología ; Cirugía ; Modelo animal ; Prevención ; Endotoxina ; Uso ; Queratitis lamelar difusa ;

Localisation / Location

INIST-CNRS, Cote INIST : 21122, 35400012492998.0060