Titre du document / Document title

Detection of nanometer-sized particles in living cells using modern fluorescence fluctuation methods

Auteur(s) / Author(s)

EDETSBERGER Michael ; GAUBITZER Erwin ; VALIC Eva ; WAIGMANN Elisabeth ; KÖHLER Gottfried ;

Résumé / Abstract

Nanosized materials are increasingly used in medicine and biotechnology but originate also from various aerosol sources. A detailed understanding of their interaction with cells is a prerequisite for specific applicationsand appraisal of hazardous effects. Fluorescence fluctuation methods are applied to follow the time-course of the translocation and distribution of fluorescent 20 nm polystyrene nanoparticles with negative surface charges in HeLa cells under almost physiological conditions. The experimental results demonstrate that singular particles enter the cell without significant contribution by endocytotic mechanisms and are distributed within the cytoplasm. Subsequently aggregation is observed, which can be blocked by cytotoxins, like Genistein and Cytochalasin B, interfering with cellular uptake processes. The observed non-active uptake is due to non-specific interactions with the cell surface and could be responsible for distribution of nanometer-sized materials in tissue.

Revue / Journal Title

Biochemical and biophysical research communications   ISSN 0006-291X   CODEN BBRCA9 

Source / Source

2005, vol. 332, n^o1, pp. 109-116 [8 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, San Diego, CA, ETATS-UNIS  (1959) (Revue)

Mots-clés d'auteur / Author Keywords

Nanoparticles ; Laser scanning microscope ; Fluorescence correlation spectroscopy: Photon counting histogramming ; Auto correlation function ; Fluorescence intensity distribution analysis ;

Localisation / Location

INIST-CNRS, Cote INIST : 8252, 35400013803466.0170