Titre du document / Document title

Cardioprotective effects of llex paraguariensis extract : evidence for a nitric oxide-dependent mechanism

Auteur(s) / Author(s)

SCHINELLA Guillermo ⁽¹⁾ ; FANTINELLI Julian C. ⁽²⁾ ; MOSCA Susan M. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Cátedra de Farmacología, Facultad de Ciencias Médicas, Universidad Nacional de La Plata, CIC, La Plata 1900, Buenos Aires, ARGENTINE
⁽²⁾ Centro de Investigaciones Cardiovasculares, Universidad Nacional de La Plata, La Plata, Buenos Aires, ARGENTINE

Résumé / Abstract

Aim: To examine the effects of an Ilex paraguariensis (Ip) extract on postischemic alterations derived from 20min of global ischemia and 30min of reperfusion. Methods: Isolated rat hearts were treated 10min before ischemia and the first 10min of reperfusion with Ip 30μg/ml. In other hearts, chelerythrine (1μM), a protein kinase C blocker, or L^G-nitro L-arginine methyl ester (L-NAME), a nitric oxide synthase inhibitor, were administered prior to Ip infusion. Left ventricular developed pressure (LVDP), +dP/dt_max, -dP/dt_max, and left ventricular end diastolic pressure (LVEDP) were used to assess myocardial function. Thiobarbituric acid reactive substances (TBARS) were measured. Results: Ip treatment produced an improvement of postichemic recovery (LVDP=96±8%; +dP/dt_max=95±10%; -dP/dt_max = 90±12% vs. 57±6%, 53±6% and 57±8%, respectively, in untreated hearts) and an attenuation of the increase of LVEDP and TBARS content. Chelerythrine did not modify and L-NAME abolished the protection induced by Ip. Conclusions: These data are the first demonstration that Ip extract attenuates the myocardial dysfunction provoked by ischemia and reperfusion and that this cardioprotection involves a diminution of oxidative damage through a nitric oxide-dependent mechanism.

Revue / Journal Title

Clinical nutrition   ISSN 0261-5614   CODEN CLNUDP 

Source / Source

2005, vol. 24, n^o3, pp. 360-366 [7 page(s) (article)] (31 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Kidlington, ROYAUME-UNI  (1982) (Revue)

Mots-clés anglais / English Keywords

Nutrition ; Mechanism ; Nitric oxide ; Extract ; Cardioprotective agent ;

Mots-clés français / French Keywords

Nutrition ; Mécanisme ; Azote monoxyde ; Extrait ; Cardioprotecteur ;

Mots-clés espagnols / Spanish Keywords

Nutrición ; Mecanismo ; Nitrógeno monóxido ; Extracto ; Cardioprotector ;

Localisation / Location

INIST-CNRS, Cote INIST : 20189, 35400012464237.0040