Titre du document / Document title

Gene array analysis of Wnt-regulated genes in C3H10T1/2 cells

Auteur(s) / Author(s)

JACKSON Amanda ⁽¹⁾ ; VAYSSIERE Béatrice ⁽¹⁾ ; GARCIA Teresa ⁽¹⁾ ; NEWELL William ⁽¹⁾ ; BARON Roland ⁽¹⁾ ; ROMAN-ROMAN Sergio ⁽¹⁾ ; RAWADI Georges ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Proskelia Pharmaceuticals, 102 route de Noisy, 93230 Romainville, FRANCE

Résumé / Abstract

Wnt/p-catenin signaling is involved in a large variety of modeling and remodeling processes including cell polarity, cell differentiation, and cell migration. Recently, a role of the Wnt pathway in bone biology has been demonstrated. However, the precise mechanism by which Wnt proteins regulate bone formation still remains to be elucidated. We have previously shown that the Wnt pathway mediates induction of alkaline phosphatase, an osteoblast differentiation marker, in the pluripotent mesenchymal cells C3H10T1/2. In the present study, we performed a genome-wide expression analysis using Affymetrix oligonucleotide chips to determine the Wnt3a-induced gene expression profile in C3H10T1/2 cells. The expression profiles of 447 Wnt3a-regulated genes, classified into distinct functional families, are presented here. Our data reveal that Wnt3a regulates several genes that are involved in osteoblast and adipocyte differentiation. Importantly, Wnt3a induces the expression of osteoprotegerin by a p-catenin dependent mechanism indicating that the Wnt pathway may also affect osteoclastogenesis. Through the analysis of our expression profiling data, we have established a TaqMan panel as a tool to rapidly compare the expression profiles of a specific set of genes induced by distinct stimuli acting in the Wnt/β-catenin pathway. Using the TaqMan panel, we have compared the gene expression profiles induced by Wnt1, Wnt2, and Wnt3a in C3H10T1/2 cells, and also by two different GSK-3β inhibitors: LiCI and SB216773. Our data show that Wnt1 and Wnt3a act in a similar manner, distinct from Wnt2. Finally, we found that Lid and SB216773 displayed different profiles in the TaqMan panel evidencing their distinct inhibitory action toward GSK-3β. Overall, data presented herein will aid further understanding of the involvement of the Wnt signaling pathway in its regulation of osteoblast and adipocyte differentiation and function and, in addition, will enhance current knowledge of the Wnt signaling pathway itself.

Revue / Journal Title

Bone   ISSN 8756-3282 

Source / Source

2005, vol. 36, n^o4, pp. 585-598 [14 page(s) (article)] (59 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, New York, NY, ETATS-UNIS  (1985) (Revue)

Mots-clés anglais / English Keywords

Enzyme ; Hydrolases ; Esterases ; Phosphoric monoester hydrolases ; Morphology ; Knowledge ; Comparative study ; Adipocyte ; Family study ; Gene expression ; Oligonucleotide ; Genome ; Differentiation ; Osteoblast ; Alkaline phosphatase ; Protein ; Mechanism ; Biology ; Bone ; Migration ; Cell differentiation ; Modeling ; Regulation(control) ; Gene ;

Mots-clés français / French Keywords

Enzyme ; Hydrolases ; Esterases ; Phosphoric monoester hydrolases ; Morphologie ; Connaissance ; Etude comparative ; Adipocyte ; Etude familiale ; Expression génique ; Oligonucléotide ; Génome ; Différenciation ; Ostéoblaste ; Alkaline phosphatase ; Protéine ; Mécanisme ; Biologie ; Os ; Migration ; Cytodifférenciation ; Modélisation ; Régulation ; Gène ;

Mots-clés espagnols / Spanish Keywords

Enzima ; Hydrolases ; Esterases ; Phosphoric monoester hydrolases ; Morfología ; Conocimiento ; Estudio comparativo ; Adipocito ; Estudio familiar ; Expresión genética ; Oligonucleótido ; Genoma ; Diferenciación ; Osteoblasto ; Alkaline phosphatase ; Proteína ; Mecanismo ; Biología ; Hueso ; Migración ; Diferenciación celular ; Modelización ; Regulación ; Gen ;

Mots-clés d'auteur / Author Keywords

C3H10T1/2 cells ; Wnt signaling ; β-catenin ;

Localisation / Location

INIST-CNRS, Cote INIST : 19041, 35400012997228.0010