Titre du document / Document title
Preparation of hypo-antigenic casein fragments having a stimulatory effect on IgA production in mouse spleen cell cultures
Auteur(s) / Author(s)ZHANG F. M.
Résumé / Abstract
Cow's casein was hydrolyzed with 13 kinds of industrial- and food-grade protease preparations of microbial, plant and animal origin. Casein phosphopeptides (CPPs) were enriched from the hydrolysates by calcium chloride aggregation at pH 7.2 followed by ethanol precipitation. The stimulatory effect of all the prepared CPPs on IgA production was higher than that of a commercially available CPP (CPP-III from tryptic casein), while the antigenic reactivity of the CPP preparations with antibody that recognized a phosphoserine-rich region, i.e., anti-p-casein (1-28) antibody, was lower than that of CPP-III. In particular, the CPP prepared from casein hydrolyzed by alkaline proteases from Aspergillus oryzae displayed an approximately 2-fold higher stimulatory effect on IgA production than CPP-III, and retained about a 100-fold lower antigenic reactivity with anti-β-casein (1-28) antibody than CPP-III did. A gel-filtration profile indicated that the molecular weight of peptides in the Aspergillus proteolytic CPP preparation seemed to be less than 3500, and that the elution position of most of the peptides corresponded to that of valinomycin (m.w. 1111). These results suggest that the CPP prepared from casein hydrolyzed by alkaline protease of Aspergillus oryzae is a hypo-antigenic peptide mixture having a strong stimulatory effect on IgA production.
Revue / Journal TitleMilchwissenschaft
Source / Source
2005, vol. 60, no
2, pp. 175-179 [5 page(s) (article)]
Langue / Language
Revue : Anglais
Editeur / Publisher
Agrar-Verlag Allgaü, Kempten, ALLEMAGNE
Localisation / Location
INIST-CNRS, Cote INIST : 4873, 35400012568227.0170
Nº notice refdoc (ud4) : 16690098