Titre du document / Document title

Toxic impact of ingested Jatropherol-I on selected enzymatic activities and the ultrastructure of midgut cells in silkworm, Bombyx mori L.

Auteur(s) / Author(s)

JING L. ⁽¹⁾ ; FANG Y. ⁽¹⁾ ; YING X. ⁽¹⁾ ; WENXING H. ⁽¹⁾ ; MENG X. ⁽¹⁾ ; SYED M. N. ⁽¹⁾ ; FANG C. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Life Science College, Sichuan University, Chengdu, CHINE

Résumé / Abstract

Jatropherol-I, a phorbol-type diterpene from Jatropha curcas seeds was found highly toxic to third instars silkworm larvae after ingestion with LC₅₀ values 0.5793, 0.2197 and 0.1578 mg/ml at 48, 72 and 120 h respectively. The acute toxicity was associated with changes in activities of several midgut enzymes and pathological changes in midgut epithelial cells. Jatropherol-I caused various fluctuations in activities of different midgut enzymes in third instars larvae of silkworm. Compared with controls, both esterase and carboxyliesterase showed significantly depressed activities at 3 h (t-test, P < 0.05) and increased activities at 24 h and rapidly decreased activities at 48 h after ingestion of 0.125 and 0.25 mg/ml of Jatropherol-I. Jatropherol-I induced two (E4 and E5) and depressed one (E2) of midgut esterase isozymes analysed 3 h after ingestion. There was no significantly different glutathione S-transferase activity between most of treated silkworm and control (t-test, P > 0.05). Activities of acetylcholinesterase fluctuated weakly in treated silkworm in 48 h. Jatropherol-I induced a gradual decline in midgut protease activities in silkworm with an obvious dose- and time-dependent effect. Jatropherol-I also caused pathological changes in midgut cells, especially in their endoplasmic reticulum. They were noted slight dilatation on 12 h exposure, while extreme dilatation, vesiculations and shedding of ribosome from membrane were observed in endoplasmic reticulum after a longer time exposure. Other pathological changes in microvilli, lysosome, mitochondria and chromatin were also observed in midgut cell of treated silkworm.

Revue / Journal Title

Journal of applied entomology   ISSN 0931-2048 

Source / Source

2005, vol. 129, n^o2, pp. 98-104 [7 page(s) (article)] (27 ref.)

Langue / Language

Anglais

Editeur / Publisher

Blackwell, Berlin, ALLEMAGNE  (1986) (Revue)

Mots-clés anglais / English Keywords

Spermatophyta ; Angiospermae ; Dicotyledones ; Euphorbiaceae ; Oil plant (vegetal) ; Bombycidae ; Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Bombyx mori ; Diterpene ; Silkworm ; Midgut ; Ultrastructure ; Enzymatic activity ; Ingestion ; Toxicity ;

Mots-clés français / French Keywords

Spermatophyta ; Angiospermae ; Dicotyledones ; Euphorbiaceae ; Plante oléagineuse ; Bombycidae ; Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Jatropha curcas ; Bombyx mori ; Diterpène ; Ver à soie ; Intestin moyen ; Ultrastructure ; Activité enzymatique ; Ingestion ; Toxicité ;

Mots-clés espagnols / Spanish Keywords

Spermatophyta ; Angiospermae ; Dicotyledones ; Euphorbiaceae ; Planta oleaginosa ; Bombycidae ; Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Bombyx mori ; Diterpeno ; Gusano de seda ; Intestino medio ; Ultraestructura ; Actividad enzimática ; Ingestión ; Toxicidad ;

Mots-clés d'auteur / Author Keywords

Bombyx mori L ; Jatropherol-I ; midgut cells ; midgut enzymes ; pathological changes ;

Localisation / Location

INIST-CNRS, Cote INIST : 6353, 35400012507282.0060