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Titre du document / Document title

New avian influenza A virus subtype combination H5N7 identified in Danish mallard ducks

Auteur(s) / Author(s)

BRAGSTAD K. ; JØRGENSEN P. H. ; HANDBERG K. J. ; MELLERGAARD S. ; CORBET S. ; FOMSGAARD A. ;

Résumé / Abstract

During the past years increasing incidences of influenza A zoonosis have made it of uppermost importance to possess methods for rapid and precise identification and characterisation of influenza A viruses. We present herea convenient one-step RT-PCR method that will amplify full-length haemagglutinin (HA) and neuraminidase (NA) directly from clinical samples and from all known subtypes of influenza A. We applied the method on samples collected in September 2003 from a Danish flock of mallards with general health problems and by this a previously undescribed influenza A subtype combination, H5N7, was identified. The HA gene showed great sequence similarity to the highly pathogenic avian influenza A virus (HPAIV) A/Chicken/Italy/312/97 (H5N2); however, the cleavage site sequence between HA1 and HA2 had a motif typical for low pathogenic avian influenza viruses (LPAIV). The full-length NA sequence was most closely related to the HPAIV A/Chicken/Netherlands/01/03 (H7N7) that infected chickens and humans in the Netherlands in 2003. Ten persons with direct or indirect contact with the Danish mallard ducks showed signs of influenza-like illness 2-3 days following the killing of the ducks, but no evidence of influence infections was detected. To our knowledge this is the first report of an H5N7 influenza A virus.

Revue / Journal Title

Virus research    ISSN  0168-1702   CODEN VIREDF 

Source / Source

2005, vol. 109, no2, pp. 181-190 [10 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Shannon, IRLANDE  (1984) (Revue)

Localisation / Location

INIST-CNRS, Cote INIST : 20313, 35400012504818.0090

Nº notice refdoc (ud4) : 16635355



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