Titre du document / Document title

Determination of modafinil in plasma and urine by reversed phase high-performance liquid-chromatography

Auteur(s) / Author(s)

SCHWERTNER Harvey A. ⁽¹⁾ ; SUK BIN KONG ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Clinical Research, Wilford Hall Medical Center, 2200 Bergquist Dr, Lackland AFB, TX 78236-5300, ETATS-UNIS
⁽²⁾ Department of Chemistry, University of the Incarnate Word, 4301 Broadway, San Antonio, TX 78209, ETATS-UNIS

Résumé / Abstract

Modafinil (Provigil) is a new wake-promoting drug that is being used for the management of excessive sleepiness in patients with narcolepsy. It has pharmacological properties similar to that of amphetamine, but without some of the side effects associated with amphetamine-like stimulants. Since modafinil has the potential to be abused, accurate drug-screening methods are needed for its analysis. In this study, we developed a high-performance liquid-chromatographic procedure (HPLC) for the quantitative analysis of modafinil in plasma and urine. (Phenylthio)acetic acid was used as an internal standard for the analysis of both plasma and urine. Modafinil was extracted from urine and plasma with ethyl acetate and ethyl acetate-acetic acid (100:1, v/v), respectively, and analyzed on a C18 reverse phase column with methanol-water-acetic acid (500:500:1, v/v) as the mobile phase. Recoveries from urine and plasma were 80.0 and 98.9%, respectively and the limit of quantitation was 0.1 μg/mL at 233 nm. Forty-eight 2-h post-dose urine samples from sham controls and from individuals taking 200 or 400 mg of modafinil were analyzed without knowledge of drug administration. All 16-placebo urine samples and all 32 2-h post-dose urine samples were correctly classified. The analytical procedure is accurate and reproducible and can be used for therapeutic drug monitoring, pharmacokinetic studies, and drug abuse screening.

Revue / Journal Title

Journal of pharmaceutical and biomedical analysis   ISSN 0731-7085   CODEN JPBADA 

Source / Source

2005, vol. 37, n^o3, pp. 475-479 [5 page(s) (article)] (9 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1983) (Revue)

Mots-clés anglais / English Keywords

CNS stimulant ; Psychotropic ; HPLC chromatography ; Reversed phase chromatography ; Urine ; Blood plasma ; Biological fluid ; Quantitative analysis ; Modafinil ;

Mots-clés français / French Keywords

Stimulant SNC ; Psychotrope ; Chromatographie HPLC ; Chromatographie phase inverse ; Urine ; Plasma sanguin ; Liquide biologique ; Analyse quantitative ; Modafinil ;

Mots-clés espagnols / Spanish Keywords

Estimulante SNC ; Psicotropo ; Cromatografía HPLC ; Cromatografía fase inversa ; Orina ; Plasma sanguíneo ; Líquido biológico ; Análisis cuantitativo ; Modafinilo ;

Mots-clés d'auteur / Author Keywords

Modafinil ; Provigil ; High-performance liquid-chromatography ; Plasma analysis ; Urine analysis ;

Localisation / Location

INIST-CNRS, Cote INIST : 19962, 35400012634052.0090