Titre du document / Document title
Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways
Auteur(s) / Author(s)
AISA Yoshinobu (1) ;
MIYAKAWA Yoshitaka (1) ;
NAKAZATO Tomonori (1) ;
SHIBATA Hideyuki (2) ;
SAITO Kaori (1) ;
IKEDA Yasuo (1) ;
KIZAKI Masahiro (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Internal Medicine, Keio University School of Medicine, Tokyo, JAPON
(2) Yakult Central Institute for Microbiological Research, Tokyo, JAPON
Résumé / Abstract
Fucoidan, a sulfated polysaccharide in brown seaweed, was found to inhibit proliferation and induce apoptosis in human lymphoma HS-Sultan cell lines. Fucoidan-induced apoptosis was accompanied by the activation of caspase-3 and was partially prevented by pretreatment with a pan-caspase inhibitor, z-VAD-FMK. The mitochondrial potential in HS-Sultan cells was decreased 24 hr after treatment with fucoidan, indicating that fucoidan induced apoptosis through a mitochondrial pathway. When HS-Sultan was treated with 100 μg/mL fucoidan for 24 hr, phosphorylation of ERK and GSK markedly decreased. In contrast, phosphorylation of p38 and Akt was not altered by treatment with fucoidan. L-Selectin and P-selectin are known to be receptors of fucoidan; however, as HS-Sultan does not express either of these selectins, it is unlikely that fucoidan induced apoptosis through them in HS-Sultan. The neutralizing antibody, Dreg56, against human L-selectin did not prevent the inhibitory effect of fucoidan on the proliferation of IM9 and MOLT4 cells, both of which express L-selectin; thus it is possible fucoidan induced apoptosis though different receptors. These results demonstrate that fucoidan has direct anticancer effects on human HS-Sultan cells through caspase and ERK pathways.
Revue / Journal Title
American journal of hematology
ISSN
0361-8609
CODEN AJHEDD
Source / Source
2005, vol. 78, n
o1, pp. 7-14 [8 page(s) (article)] (32 ref.)
Langue / Language
Anglais
Editeur / Publisher
Wiley-Liss, Hoboken, NJ, ETATS-UNIS
(1976)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 15510, 35400012705605.0020
Nº notice refdoc (ud4) : 16530759
