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Titre du document / Document title

Fucoidan induces apoptosis of human HS-Sultan cells accompanied by activation of caspase-3 and down-regulation of ERK pathways

Auteur(s) / Author(s)

AISA Yoshinobu (1) ; MIYAKAWA Yoshitaka (1) ; NAKAZATO Tomonori (1) ; SHIBATA Hideyuki (2) ; SAITO Kaori (1) ; IKEDA Yasuo (1) ; KIZAKI Masahiro (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Internal Medicine, Keio University School of Medicine, Tokyo, JAPON
(2) Yakult Central Institute for Microbiological Research, Tokyo, JAPON

Résumé / Abstract

Fucoidan, a sulfated polysaccharide in brown seaweed, was found to inhibit proliferation and induce apoptosis in human lymphoma HS-Sultan cell lines. Fucoidan-induced apoptosis was accompanied by the activation of caspase-3 and was partially prevented by pretreatment with a pan-caspase inhibitor, z-VAD-FMK. The mitochondrial potential in HS-Sultan cells was decreased 24 hr after treatment with fucoidan, indicating that fucoidan induced apoptosis through a mitochondrial pathway. When HS-Sultan was treated with 100 μg/mL fucoidan for 24 hr, phosphorylation of ERK and GSK markedly decreased. In contrast, phosphorylation of p38 and Akt was not altered by treatment with fucoidan. L-Selectin and P-selectin are known to be receptors of fucoidan; however, as HS-Sultan does not express either of these selectins, it is unlikely that fucoidan induced apoptosis through them in HS-Sultan. The neutralizing antibody, Dreg56, against human L-selectin did not prevent the inhibitory effect of fucoidan on the proliferation of IM9 and MOLT4 cells, both of which express L-selectin; thus it is possible fucoidan induced apoptosis though different receptors. These results demonstrate that fucoidan has direct anticancer effects on human HS-Sultan cells through caspase and ERK pathways.

Revue / Journal Title

American journal of hematology    ISSN  0361-8609   CODEN AJHEDD 

Source / Source

2005, vol. 78, no1, pp. 7-14 [8 page(s) (article)] (32 ref.)

Langue / Language

Anglais

Editeur / Publisher

Wiley-Liss, Hoboken, NJ, ETATS-UNIS  (1976) (Revue)

Mots-clés anglais / English Keywords

Immunopathology

;

Lymphoproliferative syndrome

;

Immunoglobulinopathy

;

Malignant hemopathy

;

Enzyme

;

Hydrolases

;

Peptidases

;

Fucoidan

;

Hematology

;

Extracellular signal-regulated protein kinase

;

Mitogen-activated protein kinase

;

Regulation(control)

;

Cysteine endopeptidases

;

Human

;

Cell death

;

Apoptosis

;

Myeloma

;

Mots-clés français / French Keywords

Immunopathologie

;

Lymphoprolifératif syndrome

;

Immunoglobulinopathie

;

Hémopathie maligne

;

Enzyme

;

Hydrolases

;

Peptidases

;

Fucoïdan

;

Caspase 3

;

Hématologie

;

Extracellular signal-regulated protein kinase

;

Mitogen-activated protein kinase

;

Régulation

;

Cysteine endopeptidases

;

Homme

;

Mort cellulaire

;

Apoptose

;

Myélome

;

Mots-clés espagnols / Spanish Keywords

Inmunopatología

;

Linfoproliferativo síndrome

;

Inmunoglobulinopatía

;

Hemopatía maligna

;

Enzima

;

Hydrolases

;

Peptidases

;

Hematología

;

Extracellular signal-regulated protein kinase

;

Mitogen-activated protein kinase

;

Regulación

;

Cysteine endopeptidases

;

Hombre

;

Muerte celular

;

Apoptosis

;

Mieloma

;

Mots-clés d'auteur / Author Keywords

fucoidan

;

apoptosis

;

multiple myeloma

;

ERK

;

caspase-3

;

Localisation / Location

INIST-CNRS, Cote INIST : 15510, 35400012705605.0020

Nº notice refdoc (ud4) : 16530759



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