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Titre du document / Document title

Residue determination of cyromazine and its metabolite melamine in chard samples by ion-pair liquid chromatography coupled to electrospray tandem mass spectrometry

Auteur(s) / Author(s)

SANCHO J. V. (1) ; IBANEZ M. (1) ; GRIMALT S. (1) ; POZO O. J. (1) ; HERNANDEZ F. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Analytical Chemistry, Experimental Sciences Department, University Jaume 1, Avda Vicente Sos Baynat, s/n, 12071, Castellón, ESPAGNE

Résumé / Abstract

A method has been developed for the sensitive and selective determination of cyromazine and its metabolite melamine in chard samples. Both compounds are small polar basic molecules, making their determination at residue levels complicated. The method involves an extraction procedure with phosphate buffer and methanol using high-speed blender, the addition of tridecafluoroheptanoic acid (TFHA) as ion-pair reagent and the injection of the five-fold diluted extract on liquid chromatography coupled to electrospray tandem mass spectrometry (LC-ESI-MS/MS). The method has been validated for chard samples, spiked at 0.05 and 0.5 mg kg-1. Quantification was carried out by using matrix-matched standards calibration and recoveries were satisfactory, with mean values for cyromazine of 103% and 93%, and relative standard deviations lower than 7%. In the case of melamine, recoveries were 89% and 86%, with relative standard deviations lower than 13%. A limit of quantification of 0.05 mg kg-1 was obtained for both compounds, with the limit of detection below 0.01 mg kg-1 The method, with very little sample handling and good sensitivity, was applied to the rapid determination of low residue levels of these compounds in chards from field residue trials. All the quality controls included during the analysis were satisfactory with average recoveries of 92% and 78% for cyromazine and melamine, respectively.

Revue / Journal Title

Analytica chimica acta    ISSN  0003-2670   CODEN ACACAM 

Source / Source

2005, vol. 530, no2, pp. 237-243 [7 page(s) (article)] (17 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1947) (Revue)

Mots-clés anglais / English Keywords

Chard

;

Melamine

;

Cyromazine

;

Detection limit

;

Standard deviation

;

Calibration standards

;

Quantitative analysis

;

Methanol

;

Phosphates

;

Sample preparation

;

Polar compound

;

Mass spectrometry MS/MS

;

Electrospray

;

Liquid chromatography

;

Ion pair

;

Metabolite

;

Residue

;

Mots-clés français / French Keywords

Bette

;

Mélamine

;

Cyromazine

;

Limite détection

;

Ecart type

;

Norme étalonnage

;

Analyse quantitative

;

Méthanol

;

Phosphate

;

Préparation échantillon

;

Composé polaire

;

Spectrométrie masse tandem

;

Electrospray

;

Chromatographie phase liquide

;

Paire ion

;

Métabolite

;

Résidu

;

Mots-clés espagnols / Spanish Keywords

Acelga

;

Ciromazina

;

Límite detección

;

Desviación típica

;

Análisis cuantitativo

;

Metanol

;

Fosfato

;

Preparación muestreo

;

Compuesto polar

;

Espectrometría masa en tándem

;

Electrospray

;

Cromatografía fase líquida

;

Par ión

;

Metabolito

;

Resíduo

;

Mots-clés d'auteur / Author Keywords

Cyromazine

;

Melamine

;

Pesticide residues

;

Vegetables

;

Liquid chromatography

;

Tandem mass spectrometry

;

Localisation / Location

INIST-CNRS, Cote INIST : 3950, 35400012673787.0100

Nº notice refdoc (ud4) : 16514561



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