Titre du document / Document title
Residue determination of cyromazine and its metabolite melamine in chard samples by ion-pair liquid chromatography coupled to electrospray tandem mass spectrometry
Auteur(s) / Author(s)
SANCHO J. V. (1) ;
IBANEZ M. (1) ;
GRIMALT S. (1) ;
POZO O. J. (1) ;
HERNANDEZ F. (1) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Analytical Chemistry, Experimental Sciences Department, University Jaume 1, Avda Vicente Sos Baynat, s/n, 12071, Castellón, ESPAGNE
Résumé / Abstract
A method has been developed for the sensitive and selective determination of cyromazine and its metabolite melamine in chard samples. Both compounds are small polar basic molecules, making their determination at residue levels complicated. The method involves an extraction procedure with phosphate buffer and methanol using high-speed blender, the addition of tridecafluoroheptanoic acid (TFHA) as ion-pair reagent and the injection of the five-fold diluted extract on liquid chromatography coupled to electrospray tandem mass spectrometry (LC-ESI-MS/MS). The method has been validated for chard samples, spiked at 0.05 and 0.5 mg kg
-1. Quantification was carried out by using matrix-matched standards calibration and recoveries were satisfactory, with mean values for cyromazine of 103% and 93%, and relative standard deviations lower than 7%. In the case of melamine, recoveries were 89% and 86%, with relative standard deviations lower than 13%. A limit of quantification of 0.05 mg kg
-1 was obtained for both compounds, with the limit of detection below 0.01 mg kg
-1 The method, with very little sample handling and good sensitivity, was applied to the rapid determination of low residue levels of these compounds in chards from field residue trials. All the quality controls included during the analysis were satisfactory with average recoveries of 92% and 78% for cyromazine and melamine, respectively.
Revue / Journal Title
Analytica chimica acta
ISSN
0003-2670
CODEN ACACAM
Source / Source
2005, vol. 530, n
o2, pp. 237-243 [7 page(s) (article)] (17 ref.)
Langue / Language
Anglais
Editeur / Publisher
Elsevier, Amsterdam, PAYS-BAS
(1947)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 3950, 35400012673787.0100
Nº notice refdoc (ud4) : 16514561
