RefDoc
Haut

Faire une nouvelle recherche
Make a new search
Lancer la recherche


Titre du document / Document title

Solid-state fermentation production of tetracycline by Streptomyces strains using some agricultural wastes as substrate

Auteur(s) / Author(s)

ASAGBRA Agnes E. (1) ; SANNI Abiodun I. (2) ; OYEWOLE Olusola B. (3) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Biotechnology Division, Federal Institute of Industrial Research, Oshodi, NIGERIA
(2) Department of Botany and Microbiology, University of Ibadan, NIGERIA
(3) Department of Food Science and Technology, University of Agriculture Abeokuta, NIGERIA

Résumé / Abstract

The ability of Streptomyces sp. OXCI, S. rimosus NRRL B2659, S. rimosus NRRL B2234, S. alboflavus NRRL B1273 S. aureofaciens NRRL B2183 and S. vendagensis ATCC 25507 to produce tetracycline using some local agricultural wastes as solid state media, were assessed. The wastes employed include peanut (groundnut) shells, corncob, corn pomace and cassava peels. Bacillus subtilis ATCC 6633 was used to assay antimicrobial activity. All the strains produced tetracycline in a solid-state fermentation process containing peanut (groundnut) as the carbohydrate source. Streptomyces sp. OXCI had the highest ability for tetracycline production with peanut shells as the substrate in solid fermentation (13.18 mg/g), followed by S. vendagensis ATCC 25507 (11.08 mg/g), S. rimosus NRRL B1679 (8.46 mg/g), S. alboflavus NRRL B1273 (7.59 mg/g), S. rimosus NRRL B2234 (6.37 mg/ g), S. aureofaciens NRRL B2183 (4.27 mg/g). Peanut (groundnut) shells were the most effective substrate (4.36 mg/ g) followed by corncob (2.64 mg/g), cassava peels (2.16 mg/g) and corn pomace (1.99 mg/g). The composition of the peanut (groundnut) shell medium optimal for tetracycline production were peanut shells 100 g, organic nitrogen (peanut meal) 10 g, (NH4)2SO4 1 g, KH2PO4 0.5 g, CaCO3 0.5 g, NaCI 0.5 g, MgSO4 . 7H2O 0.5 g, soluble starch 10 g, peanut oil 0.25 ml with initial moisture content of 65-68%, and initial pH 5.3-6.3. Substrate (1 g dry weight) was inoculated with 1.0 x 108 conidia per ml and incubated at 28-31 °C for 5-7 days, producing 13.18 mg/g of total tetracycline. Tetracycline detection started on day 3 and attained its maximum level on day 5.

Revue / Journal Title

World journal of microbiology & biotechnology    ISSN  0959-3993 

Source / Source

2005, vol. 21, no2, pp. 107-114 [8 page(s) (article)] (25 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Dordrecht, PAYS-BAS  (1990) (Revue)

Mots-clés anglais / English Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Leguminosae

;

Bacteria

;

Actinomycetes

;

Actinomycetales

;

Streptomycetaceae

;

Optimization

;

Substrate

;

Arachis hypogaea

;

Agricultural waste

;

Streptomyces

;

Tetracycline

;

Production

;

Solid substrate fermentation

;

Mots-clés français / French Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Leguminosae

;

Bactérie

;

Actinomycetes

;

Actinomycetales

;

Streptomycetaceae

;

Optimisation

;

Substrat

;

Arachis hypogaea

;

Déchet agricole

;

Streptomyces

;

Tétracycline

;

Production

;

Fermentation substrat solide

;

Mots-clés espagnols / Spanish Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Leguminosae

;

Bacteria

;

Actinomycetes

;

Actinomycetales

;

Streptomycetaceae

;

Optimización

;

Substrato

;

Arachis hypogaea

;

Desperdicio agrícola

;

Streptomyces

;

Tetraciclina

;

Producción

;

Fermentación substrato sólido

;

Mots-clés d'auteur / Author Keywords

Optimisation

;

peanut shells

;

solid-state fermentation

;

Streptomyces

;

tetracycline

;

Localisation / Location

INIST-CNRS, Cote INIST : 21001, 35400012656055.0020

Nº notice refdoc (ud4) : 16459929



Faire une nouvelle recherche
Make a new search
Lancer la recherche
Bas