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Titre du document / Document title

Cyanide distribution in human tissue, determined by GC/ECD/HS

Auteur(s) / Author(s)

CHUNSHUI ZHANG (1 2) ; HUI ZHENG (1 2) ; JIN OUYANG (2) ; SHIZHEN FENG (1) ; TAES Youri E. C. (3) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Institute of Forensic Science of Public Security Ministry of China, Beijing, CHINE
(2) Department of Chemistry, No.44, Beijing Normal University, Beijing, CHINE
(3) Institute of Forensic Science of Public Security Ministry of China, Laboratory Clinical Chemistry, University Hospital Ghent, Ghent, BELGIQUE

Résumé / Abstract

Cyanide is an important acute poison in forensic sciences. Cyanide determination is in general performed in a blood sample. We have developed a gas chromatography procedure with electron capture detector and headspace equipment (GC/ECD/ HS) for the detection of cyanide and studied cyanide distribution in various tissues in the human body, such as blood, liver, kidney, brain, urine, and stomach content. Furthermore, in a rabbit animal model, cyanide tissue distribution is determined using the developed method. A calibration curve is constructed between 0.2-3 μg/mL, R2=0.9958. Linearity is lost above 3 μg/mL. The average recovery is 84.6% (n = 8, RSD 6.39%), calculated from standard addition. The limit of detection is 0.5 ng/mL, calculated from 3 times the signal/noise signal. In humans, low cyanide concentrations (< 1 μg/mL) are observed in liver and urine, making these tissues less interesting for cyanide determination. In the rabbit animal model, tissue cyanide concentrations increase with increasing cyanide administration. A large intertissue variation is observed, with high cyanide concentration in blood, spleen, and stomach content. Spleen tissue can offer a valuable alternative for diagnosing cyanide intoxication in the absence of blood due to comparable spleen tissue concentration to blood concentration.

Revue / Journal Title

Analytical letters    ISSN  0003-2719   CODEN ANALBP 

Source / Source

2005, vol. 38, no2, pp. 247-256 [10 page(s) (article)] (9 ref.)

Langue / Language

Anglais

Editeur / Publisher

Taylor & Francis, Philadelphia, PA, ETATS-UNIS  (1967) (Revue)

Mots-clés anglais / English Keywords

Vertebrata

;

Mammalia

;

Lagomorpha

;

Rabbit

;

Urine

;

Blood

;

Poison

;

Tissue

;

Human

;

Cyanides

;

Noise

;

Detection limit

;

Standard addition method

;

Linearity

;

Trace analysis

;

Standard curve

;

Headspace

;

Forensic science

;

Electron capture detector

;

Gas chromatography

;

Mots-clés français / French Keywords

Vertebrata

;

Mammalia

;

Lagomorpha

;

Lapin

;

Urine

;

Sang

;

Toxique

;

Tissu

;

Homme

;

Cyanure

;

Bruit

;

Limite détection

;

Méthode ajout

;

Linéarité

;

Analyse trace

;

Courbe étalonnage

;

Espace tête

;

Police scientifique

;

Détecteur capture électron

;

Chromatographie phase gazeuse

;

Mots-clés espagnols / Spanish Keywords

Vertebrata

;

Mammalia

;

Lagomorpha

;

Conejo

;

Orina

;

Sangre

;

Tóxico

;

Tejido

;

Hombre

;

Cianuro

;

Ruido

;

Límite detección

;

Método añadido

;

Linearidad

;

Análisis huella

;

Curva contraste

;

Espacio cabeza

;

Ciencia forense

;

Detector captura electrón

;

Cromatografía fase gaseosa

;

Mots-clés d'auteur / Author Keywords

Cyanide

;

distribution

;

tissue

;

GC/ECD/HS

;

Localisation / Location

INIST-CNRS, Cote INIST : 13703, 35400012591385.0050

Nº notice refdoc (ud4) : 16446640



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