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Titre du document / Document title

Micro-scale analysis of lipids by far-eastern blot (TLC blot)

Auteur(s) / Author(s)

ISHIKAWA D. (1) ; TAKI T. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Cellular Technology Institute Otsuka Pharmaceutical Co., Ltd., (463-10 Kagasuno, Kawauchi-cho, Tokushima-shi 771-0192, JAPON

Résumé / Abstract

Cholesterol and glycerophospho- and sphingolipids are major constituents of the cell membrane and in certain cases function as second messengers in cell proliferation, apoptosis and cell adhesion in inflammation and tumor metastasis. To establish new methods in the lipid research, Far-eastern blot (TLC blot), was established for transferring of lipids from an high-performance thin-layer chromatography (HPTLC) plate to a polyvinyledene difluoride (PVDF) membrane within a minute. Applications of this with other methods have been studied. Far-eastern blot, makes possible the following: 1) Purification of glycosphingolipids and phospholipids. 2) Structural analysis of lipids in conjunction with direct mass spectrometry. 3) Binding study using various ligands such as antibodies, lectins, bacterium, viruses, and toxins, and 4) Enzyme reaction on membranes. Not only analysis of lipids but also metabolites of drugs and natural compounds from plants, and environmental hormones are possible by this method.

Revue / Journal Title

Nihon yukagaku kaishi    ISSN  1341-8327 

Source / Source

1998, vol. 47, no10, pp. 963-970 (24 ref.)

Langue / Language

Japonais
Revue : Divers

Editeur / Publisher

Japan Oil Chemists' Society, Tokyo, JAPON  (1996) (Revue)

Mots-clés anglais / English Keywords

Analysis method

;

Microassay

;

Thin layer chromatography

;

Vinylidene fluoride polymer

;

Chemical analysis

;

Purification

;

Structural analysis

;

Phospholipid

;

Glycolipid

;

Complex lipid

;

Sphingoglycolipid

;

Ganglioside

;

Globoside

;

Cerebroside

;

Detection

;

Chemical method

;

Enzyme immunoassay

;

Coupled method

;

Mass spectrometry

;

Mots-clés français / French Keywords

Méthode analyse

;

Microméthode

;

Chromatographie couche mince

;

Vinylidène fluorure polymère

;

Analyse chimique

;

Purification

;

Analyse structurale

;

Phospholipide

;

Glycolipide

;

Lipide complexe

;

Sphingoglycolipide

;

Ganglioside

;

Globoside

;

Cérébroside

;

Détection

;

Méthode chimique

;

Méthode immunoenzymatique

;

Méthode couplée

;

Spectrométrie masse

;

Sulfatide

;

Mots-clés espagnols / Spanish Keywords

Método análisis

;

Micrométodo

;

Cromatografía capa fina

;

Vinilideno fluoruro polímero

;

Análisis químico

;

Purificación

;

Análisis estructural

;

Fosfolípido

;

Glicolípido

;

Lípido complejo

;

Esfingoglicolípido

;

Gangliósido

;

Globósido

;

Cerebrósido

;

Detección

;

Método químico

;

Método inmunoenzimático

;

Método acoplado

;

Espectrometría masa

;

Localisation / Location

INIST-CNRS, Cote INIST : 9018, 35400007311880.0060

Nº notice refdoc (ud4) : 1638632



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