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Titre du document / Document title

A pilot study of fatty acid metabolism in oral squamous cell carcinoma

Auteur(s) / Author(s)

ZHANG Y. (1) ; GUO C. (1) ; YU G. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Oral and Maxillofacial Surgery, Peking University School of Stomatology, Beijing 100081, CHINE

Résumé / Abstract

This study was undertaken to determine the characteristics of fatty acid synthesis in squamous carcinoma cells and to assess the possibility of exploiting this synthesis pathway in anticancer therapy. Incorporation of [3H]acetate into total lipids of the TCA-83 squamous carcinoma cell line and normal gingival fibroblasts was measured. The effects of cerulenin, a specific, potent, noncompetitive inhibitor of fatty acid synthase (FAS), on growth of TCA-83 cells and normal gingival fibroblasts was determined by the MTT method, and the effect of cerulenin on apoptosis was determined by electrophoresis of cellular DNA. Incorporation of [3H]acctate into total lipids was significantly higher in TCA-83 cells (68 ± 12.7 cpm per 2 x 105 cells) than in normal gingival fibroblasts (13 ± 4.2 cpm per 2 x 105 cells; P = 0.011). Cerulenin inhibited TCA-83 cell growth in a dose-dependent manner, even at 2.5 μg/ml, a concentration at which normal gingival fibroblasts were not inhibited. After exposure to cerulenin, TCA-83 cells showed typical apoptotic DNA ladders. These results indicate that cerulenin exerts selective cytotoxic effects on squamous carcinoma cells and can induce their apoptosis. These findings suggest that targeting of fatty acid synthesis may be useful in the treatment of squamous cell carcinomas.

Revue / Journal Title

International journal of oral and maxillofacial surgery    ISSN  0901-5027   CODEN IJOSE9 

Source / Source

2005, vol. 34, no1, pp. 78-81 [4 page(s) (article)] (26 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Kidlington, ROYAUME-UNI  (1986) (Revue)

Mots-clés anglais / English Keywords

Malignant tumor

;

Oral cavity disease

;

Oral squamous cell carcinoma

;

Treatment

;

Stomatology

;

Apoptosis

;

Synthesis

;

Metabolism

;

Fatty acids

;

Surgery

;

Squamous cell carcinoma

;

Mots-clés français / French Keywords

Tumeur maligne

;

Cavité buccale pathologie

;

Carcinome épidermoïde cavité buccale

;

Traitement

;

Stomatologie

;

Apoptose

;

Synthèse

;

Métabolisme

;

Acide gras

;

Chirurgie

;

Carcinome épidermoïde

;

Mots-clés espagnols / Spanish Keywords

Tumor maligno

;

Cavidad bucal patología

;

Carcinoma epidermoide cavidad bucal

;

Tratamiento

;

Estomatología

;

Apoptosis

;

Síntesis

;

Metabolismo

;

Acido graso

;

Cirugía

;

Carcinoma epidermoide

;

Mots-clés d'auteur / Author Keywords

fatty acid synthesis

;

squamous cell carcinoma

;

cerulenin

;

apoptosis

;

Localisation / Location

INIST-CNRS, Cote INIST : 16201, 35400012605078.0120

Nº notice refdoc (ud4) : 16383479



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