Titre du document / Document title

Selective potentiometric determination of zolpidem hemitartrate in tablets and biological fluids by using polymeric membrane electrodes

Auteur(s) / Author(s)

KELANI Khadiga M. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Cairo University, Faculty of Pharmacy, Department of Analytical Chemistry, Kasr el Aini St, PO Box 11562, Cairo, EGYPTE

Résumé / Abstract

The construction and electrochemical response characteristics of 4 polymeric membrane sensors were investigated for potentiometric determination of zolpidem hemitartrate. The construction of the 4 sensors was based on the formation of ion-pair complexes between the drug cation and ionic sites in the ratio of 1:2, respectively. Two of the sensors were constructed by using ammonium reineckate or ammonium tungstate as the fixed ionic site in a polymeric matrix of polyvinyl chloride (PVC) (sensors 1 and 2). Linear responses over the concentration range of 10^-5-10^-3 M, with cationic slopes of 30 and 30.7 mV per concentration decade, were obtained by using sensors 1 and 2, respectively. The third sensor was fabricated by using PVC carboxylate (PVC-COOH). The dissociated COOH groups in the PVC-COOH act as a mediator and/or ionic site. A linear response was obtained over the concentration range of 10^-5-10^-2M with a cationic slope of 29 mV per concentration decade. Sensor 4 was fabricated by using 2,6-didodecyl-β-cyclodextrin as the ionophore, polyurethane (Tecoflex) as a polymeric matrix, and potassium tetraphenyl borate as the ionic site; it showed a linear response over the concentration range of 10^-7-10^-2 M with a cationic slope of 28.9 mV per concentration decade. The direct potentiometric determination of zolpidem hemitartrate in pure forms by using the 4 proposed sensors gave average recoveries of 98.5 ± 0.7, 99.4 ± 0.2, 100.7 ± 0.10, and 99.8 ± 0.1% for sensors 1-4 respectively. The results obtained by the proposed procedures were statistically analyzed and compared with those obtained by using a reported method. The 4 proposed sensors were also applied successfully to the determination of the drug in tablets and in biological fluids. Average recoveries obtained by using sensors 1, 2, 3, and 4 for drug assay of tablets were 99.6 ± 0.6, 100 ± 0.7, 99.7 ± 0.4, and 99.5 ± 0.8%, respectively. The presence of tablet excipients did not interfere with the determination of the drug or with the accuracy and precision of the 4 proposed methods. The methods were also used to determine the drug in the presence of its degradates and thus could be used as stability-indicating methods.

Revue / Journal Title

Journal of AOAC International   ISSN 1060-3271 

Source / Source

2004, vol. 87, n^o6, pp. 1309-1318 [10 page(s) (article)] (36 ref.)

Langue / Language

Anglais

Editeur / Publisher

AOAC International, Gaithersburg, MD, ETATS-UNIS  (1992) (Revue)

Mots-clés anglais / English Keywords

Electrochemistry ; Gabaergic agonist ; Gabaergic receptor A ; Agonist ; Sedative ; Hypnotic ; Membrane electrode ; Dosage form ; Zolpidem ; Potentiometry ; Biosensor ; Biological fluid ;

Mots-clés français / French Keywords

Electrochimie ; Stimulant gabaergique ; Récepteur gabaergique A ; Agoniste ; Sédatif ; Hypnotique ; Electrode membrane ; Forme pharmaceutique ; Zolpidem ; Potentiométrie ; Biodétecteur ; Liquide biologique ;

Mots-clés espagnols / Spanish Keywords

Electroquímica ; Estimulante gabaérgico ; Receptor gabaminérgico A ; Agonista ; Sedante ; Hipnótico ; Electrodo membrana ; Forma farmacéutica ; Zolpidem ; Potenciometría ; Biodetector ; Líquido biológico ;

Localisation / Location

INIST-CNRS, Cote INIST : 3254, 35400012128477.0060