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Titre du document / Document title

Pungency evaluation of onion cultivars from the Venezuelan West-Center region by flow injection analysis-UV-visible spectroscopy pyruvate determination

Auteur(s) / Author(s)

MARCOS Pineda (1) ; LUE-MERU Marco P. (1) ; RICARDO Rivas (1) ; MAXIMO Gallignani (2) ; MARIBEL Valero (2) ; LUIS Burguera José (2) ; MARCELA Burguera (2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Universidad Centro Occidental Lisandro Alvarado UCLA, Decanato de Agronomía, Tarabana, Cabudare, Edo. Lara, VENEZUELA
(2) Instituto Venezolano Andino de Investigaciones Químicas (IVAIQUIM), Facultad de Ciencias, Universidad de Los Andes, La Hechicera, Mérida, Edo. Mérida, VENEZUELA

Résumé / Abstract

A flow injection analysis (FIA) method was developed for the determination of pyruvate in onion cultivars (Allium cepa L.) from the West-Center region of Venezuela. The reference Schwimmer and Weston (1961) (J. Agric. Food Chem. 9 (1961) 301) Batch method was modified and adapted to FIA conditions. The formation kinetic of the 2,4-dinitrophenylhydrazine (DNPH)-pyruvate complex was evaluated at room temperature and at 37 °C. It was demonstrated the suitability of the chromopher formation at room temperature. The optimal values for the FIA parameters were: sample injection volume 3 mL, flow rate 6 mL min-1 reactor length 1.5 m, sodium hydroxide concentration 1.0 mol L-1 and hydrochloric acid concentration 0.5 mol L-1. The working calibration range was extended from 80 mg L-1 (Batch method) to 700 mg L-1 with the FIA set up. The sample dilution step is thus avoided, simplifying the whole analysis process. The pungency in representative samples of the cultivars Yellow granex 438, Ultra Hybrid and Red onion Sangre de Toro was evaluated by the flow injection analysis (FIA)-pyruvate method and the results were compared to the reference Batch pyruvate method and to the taste panel test. Non-significant differences were found at the 95% of confidence level between the FIA method and the Batch reference method. Correlation coefficient when comparing the FIA results to the taste panel test was r2 = 0.8353. Significant differences (P < 0.05) were found in the pungency of the cultivars, the Ultra Hybrid having the highest pungency. The pungency order from minor to major was: Red onion, Texas Grano 438 and Ultra Hybrid.

Revue / Journal Title

Talanta    ISSN  0039-9140   CODEN TLNTA2 

Source / Source

Congrès
ICFIA XII/JAFIA. International Conference No12, Mérida , VENEZUELA (07/12/2003)
2004, vol. 64, no 5 (310 p.)  [Document : 5 p.] (20 ref.), pp. 1299-1303 [5 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1958) (Revue)

Mots-clés anglais / English Keywords

Pyruvate

;

Hydrochloric acid

;

Sodium hydroxide

;

Organic hydrazine

;

Foodstuff

;

Cultivar

;

Onion

;

Correlation coefficient

;

Room temperature

;

Ultraviolet visible spectrometry

;

Flow injection

;

Mots-clés français / French Keywords

Pyruvate

;

Chlorhydrique acide

;

Sodium hydroxyde

;

Hydrazine organique

;

Produit alimentaire

;

Cultivar

;

Oignon

;

Coefficient corrélation

;

Température ambiante

;

Spectrométrie UV visible

;

Injection en écoulement

;

Mots-clés espagnols / Spanish Keywords

Piruvato

;

Ácido chlorhídrico

;

Sodio hidróxido

;

Hidracina orgánica

;

Producto alimenticio

;

Cultivar

;

Cebolla

;

Coeficiente correlación

;

Temperatura ambiente

;

Espectrometría UV visible

;

Inyección flujo

;

Mots-clés d'auteur / Author Keywords

Pungency

;

Onion

;

Pyruvate

;

Taste panel

;

Flow injection

;

Localisation / Location

INIST-CNRS, Cote INIST : 9221, 35400012054111.0320

Nº notice refdoc (ud4) : 16242566



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