Titre du document / Document title
Characterization of the dog Agouti gene and a nonagouti mutation in German Shepherd Dogs
Auteur(s) / Author(s)KERNS Julie A.
BERRYERE Tom G.
RUBIN Edward M.
SCHMUTZ Sheila M.
BARSH Gregory S.
Résumé / Abstract
The interaction between two genes, Agouti and Melanocortin-1 receptor (Mc1r), produces diverse pigment patterns in mammals by regulating the type, amount, and distribution pattern of the two pigment types found in mammalian hair: eumelanin (brown/black) and pheomelanin (yellow/red). In domestic dogs (Canis familiaris), there is a tremendous variation in coat color patterns between and within breeds; however, previous studies suggest that the molecular genetics of pigment-type switching in dogs may differ from that of other mammals. Here we report the identification and characterization of the Agouti gene from domestic dogs, predicted to encode a 131-amino-acid secreted protein 98% identical to the fox homolog, and which maps to chromosome CFA24 in a region of conserved linkage. Comparative analysis of the Doberman Pinscher Agouti cDNA, the fox cDNA, and 180 kb of Doberman Pinscher genomic DNA suggests that, as with laboratory mice, different pigment-type-switching patterns in the canine family are controlled by alternative usage of different promoters and untranslated first exons. A small survey of Labrador Retrievers, Greyhounds, Australian Shepherds, and German Shepherd Dogs did not uncover any polymorphisms, but we identified a single nucleotide variant in black German Shepherd Dogs predicted to cause an Arg-to-Cys substitution at codon 96, which is likely to account for recessive inheritance of a uniform black coat.
Revue / Journal TitleMammalian genome
Source / Source
2004, vol. 15, no
10, pp. 798-808 [11 page(s) (article)]
Langue / Language
Editeur / Publisher
Springer, New York, NY, ETATS-UNIS
Localisation / Location
INIST-CNRS, Cote INIST : 26645, 35400012057478.0050
Nº notice refdoc (ud4) : 16184143