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Titre du document / Document title

Cloning and purification of α-neurotoxins from king cobra (Ophiophagus hannah)

Auteur(s) / Author(s)

HE Ying-Ying (1 2) ; LEE Wei-Hui (1) ; YUN ZHANG (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Animal Toxinology, Kunming Institute of Zoology, The Chinese Academy of Sciences, 32 Fast Jiao Chang Road, Kunming 650223, Yunnan, CHINE
(2) China Three Gorge University, Yichang, 443002 Hubei, CHINE

Résumé / Abstract

Thirteen complete and three partial cDNA sequences were cloned from the constructed king cobra (Ophiophagus hannah) venom gland cDNA library. Phylogenetic analysis of nucleotide sequences of king cobra with those from other snake venoms revealed that obtained cDNAs are highly homologous to snake venom α-neurotoxins. Alignment of deduced mature peptide sequences of the obtained clones with those of other reported α-neurotoxins from the king cobra venom indicates that our obtained 16 clones belong to long-chain neurotoxins (seven), short-chain neurotoxins (seven), weak toxin (one) and variant (one), respectively. Up to now, two out of 16 newly cloned king cobra α-neurotoxins have identical amino acid sequences with CM-11 and Oh-6A/6B, which have been characterized from the same venom. Furthermore, five long-chain α-neurotoxins and two short-chain α-neurotoxins were purified from crude venom and their N-terminal amino acid sequences were determined. The cDNAs encoding the putative precursors of the purified native peptide were also determined based on the N-terminal amino acid sequencing. The purified α-neurotoxins showed different lethal activities on mice.

Revue / Journal Title

Toxicon    ISSN  0041-0101   CODEN TOXIA6 

Source / Source

2004, vol. 44, no3, pp. 295-303 [9 page(s) (article)] (1 p.1/4)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Kidlington, ROYAUME-UNI  (1962) (Revue)

Mots-clés anglais / English Keywords

Mammalia

;

Rodentia

;

Vertebrata

;

Reptilia

;

Toxin

;

Animal

;

Mouse

;

Toxicity

;

Ophidia

;

Animal origin

;

Neurotoxin

;

Purification

;

Molecular cloning

;

Mots-clés français / French Keywords

Mammalia

;

Rodentia

;

Vertebrata

;

Reptilia

;

Toxine

;

Animal

;

Souris

;

Toxicité

;

Ophidia

;

Origine animale

;

Neurotoxine

;

Purification

;

Clonage moléculaire

;

Mots-clés espagnols / Spanish Keywords

Mammalia

;

Rodentia

;

Vertebrata

;

Reptilia

;

Toxina

;

Animal

;

Ratón

;

Toxicidad

;

Ophidia

;

Origen animal

;

Neurotoxina

;

Purificación

;

Clonación molecular

;

Localisation / Location

INIST-CNRS, Cote INIST : 10409, 35400011631968.0090

Nº notice refdoc (ud4) : 16016072



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