Titre du document / Document title
HPLC separation of O-acylated flavonoids and biflavones from some species of gymnospermae
Auteur(s) / Author(s)
KRAUZE-BARANOWSKA M. (1) ;
BACZEK T. (2) ;
GŁOD D. (1) ;
KALISZAN R. (2) ;
WOLLENWEBER E. (3) ;
Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)
(1) Department of Pharmacognosy, Medical University of Gdańnsk Gen. J. Hallera 107 str., 80-416 Gdańsk, POLOGNE
(2) Department of Pharmacology and Biopharmacy, Medical University of Gdańsk, Gen. J. Hallera 107 str., 80-416 Gdańsk, POLOGNE
(3) Institut für Botanik der TU Darmstadt, Schnittspahnstr. 3, 64287 Darmstadt, ALLEMAGNE
Résumé / Abstract
Flavonoids present in the extracts from leaves of Pseudotsuga menziesii (Pinaceae), Ginkgo biloba (Ginkgoaceae) and Podocarpus dacrydioides (Podocarpaceae) were separated by use of the reversed phase HPLC method. The analysed compounds belong to different groups of flavonoids - biflavones (amentoflavone, bilobetin, 5'-methoxybilobetin, podocarpusflavone A, sequoiaflavone, podocarpusflavone B, ginkgetin, isoginkgetin, sciadopitysin, kayaflavone, hinokiflavone, 2,3-dihydrosciadopitysin, 2,3-dihydroisoginkgetin), O-acylated flavonol glycosides (daglesiosides I, II, III, IV, trans-tiliroside, trans-ditiliroside), flavonol O-glycosides (astragalin, isoquercetin) and flavonol aglycones (kaempferol, quercetin, isorhamnetin). The conditions for flavonoid separation were optimized using various RP-18 columns. The chromatographic resolution was performed with isocratic or gradient elution - optimized by Drylab program or by traditional trial-and-error method, depending on the composition of flavonoid complex.
Revue / Journal Title
Chromatographia
ISSN
0009-5893
CODEN CHRGB7
Source / Source
2004, vol. 60, n
o1-2, pp. 9-15 [7 page(s) (article)] (28 ref.)
Langue / Language
Anglais
Editeur / Publisher
Springer, Wiesbaden, ALLEMAGNE
(1968)
(Revue)
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Localisation / Location
INIST-CNRS, Cote INIST : 8489, 35400011617215.0020
Nº notice refdoc (ud4) : 15995625
