Titre du document / Document title

Selection of enterococci for potential canine probiotic additives

Auteur(s) / Author(s)

STROMPFOVA Viola ⁽¹⁾ ; LAUKOVA Andrea ⁽¹⁾ ; OUWEHAND Arthur C. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Institute of Animal Physiology, Slovak Academy of Sciences, Šoltésovej 4-6, 04001 Košice, SLOVAQUIE
⁽²⁾ Department of Biochemistry and Food Chemistry, University of Turku, Vatselanjatu 2, 20014 Turku, FINLANDE

Résumé / Abstract

Enterococci are important inhabitants of animal intestine and are widely used in probiotic products. A potentially successful probiotic strain is expected to have several desirable properties in order to be able to exert its beneficial effects. Forty enterococcal isolates from dog faeces were tested for characters believed to be important for probiotic strains; bacteriocin production, resistance or tolerance to antibiotics, low pH, bile tolerance and adhesive activity. The total count of enterococci was found to be 3.3-7.3 log₁₀ CFU/g of faeces. Most identified strains were Enterococcus faecium. All strains were sensitive to vancomycin, ampicillin, penicillin and chloramphenicol. Thirty-three percentage of strains were resistant to erythromycin and 28% to tetracycline. Among 40 isolates, 75% showed a broad inhibitory spectrum only against Gram-positive indicator bacteria. Seven strains with broad bacteriocin activity were selected for further assays. In the presence of 1% bile, the survival rate of selected strains ranged between 72 and 98%. Survival of strains at pH 3.0 was found in the range between 76 and 87% after 3 h. The adhesion of the tested strains to intestinal mucus ranged from 4 to 11 % for canine mucus and from 5 to 8% for human mucus. E. faecalis EE4 and E. faecium EF01 showed the best probiotic properties. It indicates that they could be used as new candidate probiotic strains after in vivo testing.

Revue / Journal Title

Veterinary microbiology   ISSN 0378-1135   CODEN VMICDQ 

Source / Source

2004, vol. 100, n^o1-2, pp. 107-114 [8 page(s) (article)] (30 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier Science, Amsterdam, PAYS-BAS  (1976) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Carnivora ; Fissipedia ; Bacteria ; Micrococcales ; Streptococcaceae ; Veterinary ; Microbiology ; Antimicrobial agent ; Bile acid ; Antibiotic ; Adhesion ; Identification ; Probiotic ; Dog ; Enterococcus ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Carnivora ; Fissipedia ; Bactérie ; Micrococcales ; Streptococcaceae ; Vétérinaire ; Microbiologie ; Antimicrobien ; Acide biliaire ; Antibiotique ; Adhérence ; Identification ; Probiotique ; Chien ; Enterococcus ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Carnivora ; Fissipedia ; Bacteria ; Micrococcales ; Streptococcaceae ; Veterinario ; Microbiología ; Antimicrobiano ; Acido biliar ; Antibiótico ; Adherencia ; Identificación ; Probioticó ; Perro ; Enterococcus ;

Mots-clés d'auteur / Author Keywords

Enterococcus sp ; Probiotic ; Identification ; Adhesion ; Antibiotic ; Acid and bile tolerance ; Antimicrobial substances ;

Localisation / Location

INIST-CNRS, Cote INIST : 16884, 35400011003689.0120