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Titre du document / Document title

Analysis of squalene synthase expression during the development of Ganoderma lucidum

Auteur(s) / Author(s)

ZHAO M. W. (1 2) ; ZHONG J. Y. (1) ; LIANG W. Q. (2) ; WANG N. (3) ; CHEN M. J. (3) ; ZHANG D. B. (2) ; PAN Y. J. (3) ; JONG S. C. (4) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Microbiology Department, Nanjing Agricultural University, Key Laboratory of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, Nanjing 210095, CHINE
(2) Agro-Biotech Research Center, Agricultural Academy of Shanghai, Key Laboratory of Agriculture Genetics and Breeding of Shanghai, Shanghai 201106, CHINE
(3) Agricultural Academy of Shanghai, Key Laboratory of Edible Fungus Genetics and Breeding Edible Fungi Institute, Ministry of Agriculture, Key Laboratory of Agriculture Genetics and Breeding of Shanghai, CHINE
(4) American Type Culture Collection, 10801 University Boulevard, Manassas, VA 20110-2209, ETATS-UNIS

Résumé / Abstract

The medicinal properties of Ganoderma lucidum have been recognized in China for many centuries. Active pharmaceutical components include triterpenes. To elucidate the molecular regulation of triterpene biosynthesis in this mushroom, a 57-base pair encoding the fourth conserved domain SQ-4 (SMGLFLQKTNIIRDYNEDL) of squalene synthase was synthesized and cloned into the expression vector pET-32a(+). The recombinant fusion protein induced by IPTG (isopropyl-β-D-thiogalactopyranoside) was overexpressed in the Escherichia coli. Using the purified recombinant fusion protein of 20.9 kDa, a specific polyclonal antibody was obtained from immunized rabbit. Expression of squalene synthase at different development stages of Ganoderma lucidum was analyzed.

Revue / Journal Title

Journal of microbiology and biotechnology    ISSN  1017-7825 

Source / Source

2004, vol. 14, no1, pp. 116-120 [5 page(s) (article)] (26 ref.)

Langue / Language

Anglais

Editeur / Publisher

Korean Society for Applied Microbiology, Seoul, COREE, REPUBLIQUE DE  (1991) (Revue)

Mots-clés anglais / English Keywords

Thallophyta

;

Fungi

;

Basidiomycetes

;

Enzyme

;

Transferases

;

Ganoderma

;

Farnesyl-diphosphate farnesyltransferase

;

Mots-clés français / French Keywords

Thallophyta

;

Fungi

;

Basidiomycetes

;

Enzyme

;

Transferases

;

Ganoderma

;

Farnesyl-diphosphate farnesyltransferase

;

Mots-clés espagnols / Spanish Keywords

Thallophyta

;

Fungi

;

Basidiomycetes

;

Enzima

;

Transferases

;

Ganoderma

;

Farnesyl-diphosphate farnesyltransferase

;

Mots-clés d'auteur / Author Keywords

Expression characteristics

;

Ganoderma lucidum

;

squalene synthase

;

triterpene

;

Localisation / Location

INIST-CNRS, Cote INIST : 26482, 35400011667202.0170

Nº notice refdoc (ud4) : 15520513



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