Titre du document / Document title
Slow growth in vitro preservation of African violet (Saintpaulia ionantha Wendl.)
Auteur(s) / Author(s)MOGES A. D.
KARAM N. S.
SHIBLI R. A.
Résumé / Abstract
In vitro preservation of African violet (Saintpaulia ionantha Wendl.) via slow growth was evaluated using different osmotic agents and temperatures. Microshoots were excised from in vitro plantlets and cultured on a hormone-free MS medium containing different concentrations of sucrose (0, 0.09, 0.18, 0.26 or 0.35 M), mannitol or sorbitol (0, 0.16, 0.33, 0.49 or 0.66 M). Other microshoots were cultured on a hormone-free MS medium supplemented with 0.09 M sucrose and incubated at 2, 10, or 24°C in the dark. Results indicated that preservation of microshoots on a medium containing 0.18 M sucrose or 0.16 M mannitol or sorbitol under 16 hr light was able to decrease shoot growth and maintain explant quality for 12 weeks. Preservation of microshoots at 10°C in the dark was effective in decreasing the growth and maintaining explants in a good condition for six weeks.
Revue / Journal TitleAdvances in horticultural science
Source / Source
4, pp. 223-230 [8 page(s) (article)]
Langue / Language
Editeur / Publisher
University of Florence, Department of Horticulture, Firenze, ITALIE
Localisation / Location
INIST-CNRS, Cote INIST : 20764, 35400011653079.0070
Nº notice refdoc (ud4) : 15505691