Titre du document / Document title

Slow growth in vitro preservation of African violet (Saintpaulia ionantha Wendl.)

Auteur(s) / Author(s)

MOGES A. D. ; KARAM N. S. ; SHIBLI R. A. ;

Résumé / Abstract

In vitro preservation of African violet (Saintpaulia ionantha Wendl.) via slow growth was evaluated using different osmotic agents and temperatures. Microshoots were excised from in vitro plantlets and cultured on a hormone-free MS medium containing different concentrations of sucrose (0, 0.09, 0.18, 0.26 or 0.35 M), mannitol or sorbitol (0, 0.16, 0.33, 0.49 or 0.66 M). Other microshoots were cultured on a hormone-free MS medium supplemented with 0.09 M sucrose and incubated at 2, 10, or 24°C in the dark. Results indicated that preservation of microshoots on a medium containing 0.18 M sucrose or 0.16 M mannitol or sorbitol under 16 hr light was able to decrease shoot growth and maintain explant quality for 12 weeks. Preservation of microshoots at 10°C in the dark was effective in decreasing the growth and maintaining explants in a good condition for six weeks.

Revue / Journal Title

Advances in horticultural science    ISSN  0394-6169 

Source / Source

2003, n^o4, pp. 223-230 [8 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

University of Florence, Department of Horticulture, Firenze, ITALIE  (1987) (Revue)

Localisation / Location

INIST-CNRS, Cote INIST : 20764, 35400011653079.0070