Titre du document / Document title

Evaluation of phytochemical and antimicrobial properties of leaf extract of Tapinanthus sessilifolius (P. Beauv) van Tiegh

Auteur(s) / Author(s)

TARFA Florence D. ; OBODOZIE Obiageri O. ; MSHELIA Emmanuel ; IBRAHIM Kolo ; TEMPLE V. J. ;

Résumé / Abstract

Leaf extracts of T. sessilifolius growing on five different host plants (Psidium guajava, Citrus lemon, Vernonia amygdalina, Persea americana and Jatropa curcas) were evaluated for antimicrobial activity of the plant. Powdered leaves of T. sessilifolius collected from each host plant was divided into two portions. One portion was used for aqueous infusion and the other portion was successively extracted with hexane, ethylacetate and methanol. Infusion of aqueous extract of powdered leaves did not show antimicrobial effect even at the concentration of 1000 and 2000μg/ml on test microorganisms (Staph. aureus, E. coli, Bacillus subtilis, Pseudomonas aeruginosa and Candida albicans). However in broth culture, methanolic and hexane extract had MIC range of 62.5-500μg/ml and ethylacetate extract had 250-500 pg/ml. Phytochemical screening of leaf samples of T. sessilifolius collected from different host plants showed positive test for hydrolysable tannins, saponins, flavonoids, terpenes, cardiac glycoside, reducing sugars and proteins.LD⁵⁰ concentration was found to be > 1.500 mg/kg for samples from P. guajava; 489.89 mg/kg for J. curcas and C. lemon; and 692 mg/kg for V. amydalina in mice.

Revue / Journal Title

Indian journal of experimental biology   ISSN 0019-5189   CODEN IJEBA6 

Source / Source

2004, vol. 42, n^o3, pp. 326-329 [4 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Council of Scientific & Industrial Research, New Delhi, INDE  (1963) (Revue)

Localisation / Location

INIST-CNRS, Cote INIST : 2364 C, 35400011500981.0160