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Titre du document / Document title

Molecular structures of Fructans from Agave tequilana Weber var. azul

Auteur(s) / Author(s)

LOPEZ Mercedes G. (1) ; MANCILLA-MARGALLI Norma A. (1) ; MENDOZA-DIAZ Guillermo (2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Departamento de Biotecnologia y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Apartado Postal 629, Irapuato, Gto., 36500 México, MEXIQUE
(2) Facultad de Química, Universidad de Guanajuato, Noria Alta s/n, Guanajuato, Gto., 36050 México, MEXIQUE

Résumé / Abstract

Agave plants utilize crassulacean acid metabolism (CAM) for CO2 fixation. Fructans are the principal photosynthetic products generated by agave plants. These carbohydrates are fructose-bound polymers frequently with a single glucose moiety. Agave tequilana Weber var. azul is an economically important CAM species not only because it is the sole plant allowed for tequila production but because it is a potential source of prebiotics. Because of the large amounts of carbohydrates in A. tequilana, in this study the molecular structures of its fructans were determined by fructan derivatization for linkage analysis coupled with gas chromatography-mass spectrometry (GC-MS), nuclear magnetic resonance (NMR), and matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS). Fructans were extracted from 8-year-old A. tequilana plants. The linkage types present in fructans from A. tequilana were determined by permethylation followed by reductive cleavage, acetylation, and finally GC-MS analysis. Analysis of the degree of polymerization (DP) estimated by 1H NMR integration and 13C NMR and confirmed by MALDI-TOF-MS showed a wide DP ranging from 3 to 29 units. All of the analyses performed demonstrated that fructans from A. tequilana consist of a complex mixture of fructooligosaccharides containing principally β(2 → 1) linkages, but also β(2 → 6) and branch moieties were observed. Finally, it can be stated that fructans from A. tequilana Weber var. azul are not an inulin type as previously thought.

Revue / Journal Title

Journal of agricultural and food chemistry    ISSN  0021-8561   CODEN JAFCAU 

Source / Source

2003, vol. 51, no27, pp. 7835-7840 [6 page(s) (article)] (29 ref.)

Langue / Language

Anglais

Editeur / Publisher

American Chemical Society, Washington, DC, ETATS-UNIS  (1953) (Revue)

Mots-clés anglais / English Keywords

Alcoholic beverage

;

America

;

Central America

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Agavaceae

;

Succulent plant

;

Mexico

;

Tequila

;

Time of flight spectrometer

;

Matrix assisted laser desorption ionization

;

Polymerization

;

Molecular structure

;

Agave

;

Prebiotics

;

Mots-clés français / French Keywords

Boisson alcoolisée

;

Amérique

;

Amérique Centrale

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Agavaceae

;

Agave tequilana

;

Fructane

;

Plante succulente

;

Mexique

;

Tequila

;

Spectromètre temps vol

;

MALDI

;

Polymérisation

;

Structure moléculaire

;

Agave

;

Substance prébiotique

;

Mots-clés espagnols / Spanish Keywords

Bebida alcohólica

;

America

;

America central

;

Spermatophyta

;

Angiospermae

;

Monocotyledones

;

Agavaceae

;

Planta suculenta

;

Méjico

;

Tequila

;

Espectrómetro para el tiempo de vuelo de los electrones

;

MALDI

;

Polimerización

;

Estructura molecular

;

Agave

;

Prebioticos

;

Mots-clés d'auteur / Author Keywords

Agave tequilana

;

fructans

;

linkage

;

permethylated alditol acetates

;

degree of polymerization (DP)

;

matrix-assisted laser desorption time-of-flight mass spectrometry (MALDI-TOF-MS)

;

Localisation / Location

INIST-CNRS, Cote INIST : 7332, 35400011908606.0010

Nº notice refdoc (ud4) : 15390449



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