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Titre du document / Document title

Gc protein-derived macrophage activating factor (GcMAF): Isoelectric focusing pattern and tumoricidal activity

Auteur(s) / Author(s)

SAHARUDDIN BIN MOHAMAD (1) ; NAGASAWA Hideko (1) ; SASAKI Hideyuki (1) ; UTO Yoshihiro (1) ; NAKAGAWA Yoshinori (2) ; KAWASHIMA Ken (3) ; HORI Hitoshi (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Biological Science and Technology, Faculty of Engineering, The University of Tokushima, Minamijyosanjimacho-2, Tokushima, 770-8506, JAPON
(2) Hayashibara Biochemical Laboratory, Kanko-Shikiso Institute Inc., Okayama, 700-0907, JAPON
(3) Meneki Bunseki Kenkyu Center Co. (MBK), Tsuyama, Okayama, 708-0806, JAPON

Résumé / Abstract

Background: Gc protein is the precursor for Gc protein-derived macrophage activating factor (GcMAF), with three phenotypes: Gc1f, Gcls and Gc2, based on its elecrophoretic mobility. The difference in elecrophoretic mobility is because of the difference in its posttranslational sugar moiety composition. Materials and Methods: We compared the difference between Gc protein and GcMAF elecrophoretic mobility using the isoelectric focusing (IEF) method. The tumoricidal activity of GcMAF-treated macrophage was evaluated after coculture with L-929 cell. The tumoricidal mechanism was investigated using TNF bioassay and nitric oxide (NO) release. Results: The difference in Gc protein and GcMAF elecrophoretic mobility was detected. The tumoricidal activity of GcMAF-treated macrophage was detected, but no release of TNF and NO was detected. Conclusion: The difference of isoelectric focusing mobility in Gc protein and GcMAF would be useful to develop a GcMAF detection method. GcMAF increased macrophage tumoricidal activity but TNF and NO release were not involved in the mechanism.

Revue / Journal Title

Anticancer research    ISSN  0250-7005 

Source / Source

Congrès
Annual Meeting of the Society of Biotherapeutic Approaches No6, Fukuoka , JAPON (07/12/2002)
2003, vol. 23, no 6A (119 p.)  [Document : 7 p.] (21 ref.), pp. 4451-4457 [7 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

International Institute of Anticancer Research, Attiki, GRECE  (1980) (Revue)

Mots-clés anglais / English Keywords

Cytokine

;

Cancerology

;

Nitric oxide

;

Activity

;

Isoelectric focusing

;

Macrophage

;

Protein

;

Tumor necrosis factor

;

Mots-clés français / French Keywords

Cytokine

;

Cancérologie

;

Azote monoxyde

;

Activité

;

Focalisation isoélectrique

;

Macrophage

;

Protéine

;

Facteur nécrose tumorale

;

Mots-clés espagnols / Spanish Keywords

Citoquina

;

Cancerología

;

Nitrógeno monóxido

;

Actividad

;

Focalización isoeléctrica

;

Macrófago

;

Proteína

;

Factor necrosis tumoral

;

Mots-clés d'auteur / Author Keywords

Gc protein-derived macrophage activating factor (GcMAF)

;

isoelectric focusing (IEF)

;

tumoricidal

;

tumor necrosis factor (TNF)

;

nitric oxide (NO)

;

Localisation / Location

INIST-CNRS, Cote INIST : 19426, 35400011600633.0110

Nº notice refdoc (ud4) : 15348962



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