Titre du document / Document title

A fish oil diet does not reverse insulin resistance despite decreased adipose tissue TNF-α protein concentration in AapoE-3*Leiden mice¹

Auteur(s) / Author(s)

MUURLING Martin ^{(1 2)} ; MENSINK Ronald P. ⁽²⁾ ; PIJL Hanno ⁽³⁾ ; ROMIJN Johannes A. ⁽⁴⁾ ; HAVEKES Louis M. ^{(1 3)} ; VOSHOL Peter J. ⁽⁴⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ TNO-Prevention and Health, Gaubius Laboratory Leiden, 2301 CE Leiden, PAYS-BAS
⁽²⁾ Department of Human Biology/NUTRIM, Maastricht University, 6200 MD Maastricht, PAYS-BAS
⁽³⁾ Department of Cardiology and Internal Medicine, Leiden University Medical Center, 2300 RC Leiden, PAYS-BAS
⁽⁴⁾ Department of Endocrinology and Diabetes, Leiden University Medical Center, 2300 RC Leiden, PAYS-BAS

Résumé / Abstract

Dietary interventions with fish oil have been found to protect against the development of high-fat diet-induced insulin resistance and to decrease the expression of tumor necrosis factor (TNF)-α. However, the effect of fish oil administration on preexisting insulin resistance is subject to debate. In the present study, we examined the mechanism by which fish oil affects preexisting insulin resistance. High fat diet-induced insulin-resistant ApoE*3-Leiden transgenic mice were treated for 10 wk as follows: 1) high fat diet (control group), 2) high fat diet with 3 g/100 g fish oil and 3) high fat diet but food intake restricted to 75% of the ad libitum food intake. We measured plasma glucose, insulin, free fatty acids (FFA) and triglyceride (TG) levels throughout the study. After the 10-wk dietary intervention period we performed hyperinsulinemic euglycemic analyses and measured insulin sensitivity and FFA turnover. Furthermore, we then determined the VLDL-TG production rate and TNF-a protein expression in white adipose tissue (WAT). Compared with control mice, the insulin sensitivity of mice treated with fish oil was not affected, whereas it was improved (P < 0.05) for energy-restricted mice. FFA turnover was unaffected in both fish oil-treated and energy-restricted mice. Compared with controls, hepatic VLDL-TG production was lower (P < 0.05) with fish oil feeding but greater with energy restriction (P < 0.05). Interestingly, the level of TNF-a protein in WAT was lower (P < 0.05) in both groups. We conclude that partial replacement of saturated fat by fish oil does not improve preexisting high fat diet-induced insulin resistance, although it lowers TNF-α protein levels in WAT. J. Nutr. 133: 3350-3355, 2003.

Revue / Journal Title

The Journal of nutrition   ISSN 0022-3166   CODEN JONUAI 

Source / Source

2003, vol. 133, n^o11, pp. 3350-3355 [6 page(s) (article)] (36 ref.)

Langue / Language

Anglais

Editeur / Publisher

American Society for Nutritional Sciences, Bethesda, MD, ETATS-UNIS  (1928) (Revue)

Mots-clés anglais / English Keywords

Feeding behavior ; Lipids ; Pancreatic hormone ; Hemopathy ; Coagulopathy ; Cytokine ; Feeding ; Insulin resistance ; Sensitivity ; Insulin ; Hypercoagulability ; Thrombophilia ; Concentration ; Protein ; Tumor necrosis factor α ; Adipose tissue ; Diet ; Fish oil ;

Mots-clés français / French Keywords

Comportement alimentaire ; Lipide ; Hormone pancréatique ; Hémopathie ; Coagulopathie ; Cytokine ; Alimentation ; Résistance insuline ; Sensibilité ; Insuline ; Hypercoagulabilité ; Thrombophilie ; Concentration ; Protéine ; Facteur nécrose tumorale α ; Tissu adipeux ; Régime alimentaire ; Huile poisson ;

Mots-clés espagnols / Spanish Keywords

Conducta alimenticia ; Lípido ; Hormona pancreática ; Hemopatía ; Coagulopatía ; Citoquina ; Alimentación ; Resistancia insulina ; Sensibilidad ; Insulina ; Hipercoagulabilidad ; Trombofilia ; Concentración ; Proteína ; Factor necrosis tumoral α ; Tejido adiposo ; Régimen alimentario ; Aceite pescado ;

Mots-clés d'auteur / Author Keywords

·fish oil ; insulin resistance ; insulin sensitivity ; free fatty acid metabolism ; tumor necrosis factor-a ;

Localisation / Location

INIST-CNRS, Cote INIST : 2042, 35400011874097.0060