Titre du document / Document title

Comparative study on the susceptibility of cutworms (Lepidoptera: Noctuidae) to Agrotis segetum nucleopolyhedrovirus and Agrotis ipsilon nucleopolyhedrovirus

Auteur(s) / Author(s)

EL-SALAMOUNY Said ^{(1 2)} ; LANGE Martin ⁽¹⁾ ; JUTZI Manfred ⁽¹⁾ ; HUBER Jürg ⁽³⁾ ; JEHLE Johannes A. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ State Education and Research Center for Agriculture, Viticulture and Horticulture (SLFA), Biotechnological Crop Protection, Breitenweg 71, 67435 Neustadt/Weinstrasse, ALLEMAGNE
⁽²⁾ Department of Economic Entomology and Pesticides, Faculty of Agriculture, Cairo University, 12613 Ciza, EGYPTE
⁽³⁾ Federal Biological Research Center for Agriculture and Forestry (BBA), Institute for Biological Control, Heinrichstr. 243, 64287 Darmstadt, ALLEMAGNE

Résumé / Abstract

The common cutworm (Agrotis segetum) and the black cutworm (Agrotis ipsilon) are serious soil pests of many vegetable and field crops all over the world. We have demonstrated the cross-infectivity of two baculoviruses, A. segetum nucleopolyhedrovirus (AgseNPV) and A. ipsilon nucleopolyhedrovirus (AgipNPV) for these two insect pests. The susceptibility of A. segetum to AgipNPV was confirmed by DNA restriction endonuclease analyses of DNA isolated from virus harvested from infected A. segetum larvae. For an initial comparison of both viruses, partial polyhedrin sequences were amplified by PCR, cloned, and sequenced. Both viruses shared a very similar polyhedrin gene sequence resulting in only three amino acid substitutions. Phylogenetic analyses clearly demonstrated that both viruses belong to NPV group II and are most closely related to a Glade consisting of Spodoptera exigua NPV, Spodoptera frugiperda NPV, and Spodoptera littoralis NPV. Since AgipNPV shows high virulence for both cutworm species, it appears to be a suitable candidate as a single biological control agent of A. segetum and A. ipsilon.

Revue / Journal Title

Journal of invertebrate pathology   ISSN 0022-2011   CODEN JIVPAZ 

Source / Source

2003, vol. 84, n^o2, pp. 75-82 [8 page(s) (article)] (32 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1965) (Revue)

Mots-clés anglais / English Keywords

Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Noctuidae ; Host virus relation ; Biological control ; Taxonomy ; Virus ; Baculoviridae ; Agrotis ipsilon ; Agrotis segetum ; Nucleotide sequence ; Phylogeny ; Microbiological control ; Nucleopolyhedrovirus ; Sensitivity resistance ; Pest ; Larva ; Interspecific comparison ;

Mots-clés français / French Keywords

Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Noctuidae ; Relation hôte virus ; Lutte biologique ; Systématique ; Virus ; Baculoviridae ; Agrotis ipsilon ; Agrotis segetum ; Séquence nucléotide ; Phylogenèse ; Lutte microbiologique ; Nucleopolyhedrovirus ; Sensibilité résistance ; Déprédateur ; Larve ; Comparaison interspécifique ;

Mots-clés espagnols / Spanish Keywords

Invertebrata ; Arthropoda ; Insecta ; Lepidoptera ; Noctuidae ; Relación huesped virus ; Lucha biológica ; Sistemática ; Virus ; Baculoviridae ; Agrotis ipsilon ; Agrotis segetum ; Secuencia nucleótido ; Filogénesis ; Lucha microbiológica ; Nucleopolyhedrovirus ; Sensibilidad resistencia ; Plaga ; Larva ; Comparación interespecífica ;

Mots-clés d'auteur / Author Keywords

Agrotis segetum ; Agrotis ipsilon: Agrotis segetum nucleopolyhedrovirus ; Agrotis ipsilon nucleopolyhedrovirus ; Cross-transmission ; Polyhedrin sequence ; Phylogeny ; Biological control ;

Localisation / Location

INIST-CNRS, Cote INIST : 9246, 35400011877777.0020