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Titre du document / Document title

Analysis of clinical isolates of Propionibacterium acnes by optimised RAPD

Auteur(s) / Author(s)

PERRY Alexandra L. (1) ; WORTHINGTON Tony (2) ; HILTON Anthony C. (1) ; LAMBERT Peter A. (1) ; STIRLING Alistair J. (3) ; ELLIOTT Tom S. J. (2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Microbiology Research, Life and Health Sciences, Aston University, Birmingham B4 7ET, ROYAUME-UNI
(2) Department of Clinical Microbiology, Queen Elizabeth Hospital, University Hospital Birmingham NHS Trust, Edgbaston, Birmingham B15 2TH, ROYAUME-UNI
(3) Royal Orthopaedic Hospital, Northfield, Birmingham B31 2AP, ROYAUME-UNI

Résumé / Abstract

Random amplification of polymorphic DNA (RAPD) was evaluated as a genotypic method for typing clinical strains of Propionibacterium acnes. RAPD can suffer from problems of reproducibility if parameters are not standardised. In this study the reaction conditions were optimised by adjusting template DNA concentration and buffer constituents. All isolates were typeable using the optimised RAPD protocol which was found to be highly discriminatory (Simpson's diversity index, 0.98) and reproducible. Typing of P. acnes by optimised RAPD is an invaluable tool for the epidemiological investigation of P. acnes for which no other widely accepted method currently exists.

Revue / Journal Title

FEMS microbiology letters    ISSN  0378-1097   CODEN FMLED7 

Source / Source

2003, vol. 228, no1, pp. 51-55 [5 page(s) (article)] (19 ref.)

Langue / Language

Anglais

Editeur / Publisher

Wiley-Blackwell, Oxford, ROYAUME-UNI  (1977) (Revue)

Mots-clés anglais / English Keywords

Skin disease

;

Bacteria

;

Actinomycetes

;

Propionibacteriaceae

;

Acne

;

Genotype

;

Molecular epidemiology

;

Amplification

;

Random amplified polymorphic DNA marker

;

Clinical isolate

;

Propionibacterium acnes

;

Mots-clés français / French Keywords

Peau pathologie

;

Bactérie

;

Actinomycetes

;

Propionibacteriaceae

;

Acné

;

Génotype

;

Epidémiologie moléculaire

;

Amplification

;

Marqueur RAPD

;

Isolat clinique

;

Propionibacterium acnes

;

Mots-clés espagnols / Spanish Keywords

Piel patología

;

Bacteria

;

Actinomycetes

;

Propionibacteriaceae

;

Acné

;

Genotipo

;

Epidemiología molecular

;

Amplificación

;

Marcador RAPD

;

Aislado clinico

;

Propionibacterium acnes

;

Mots-clés d'auteur / Author Keywords

Propionibacierium acnes

;

Random amplification of polymorphic DNA

;

Molecular typing

;

Localisation / Location

INIST-CNRS, Cote INIST : 17567 A, 35400011486660.0080

Nº notice refdoc (ud4) : 15247051



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