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Titre du document / Document title

Active oxygen species generated from photoexcited fullerene (C60) as potential medicines: O2-○ versus 1O2

Auteur(s) / Author(s)

YAMAKOSHI Yoko (1) ; UMEZAWA Naoki (2) ; RYU Akemi (3) ; ARAKANE Kumi (3) ; MIYATA Naoki (1) ; GODA Yukihiro (1) ; MASUMIZU Toshiki (4) ; NAGANO Tetsuo (2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Division of Organic Chemistry, National Institute of Health Sciences, 1-18-1 Kamiyoga, Setagaya, Tokyo 158-8501, JAPON
(2) Graduate School of Pharmaceutical Sciences, The University of Tokyo 7-3-1 Hongo, Bunkyo, Tokyo 113-0033, JAPON
(3) Research Laboratory, KOSÉ Corporation, 1-18-4 Azusawa, Itabashi, Tokyo 174-0051, JAPON
(4) Application and Research Center, JEOL, 3-1-2 Musashino, Akishima, Tokyo 196-8558, JAPON

Résumé / Abstract

To characterize fullerenes (C60 and C70) as photosensitizers in biological systems, the generation of active oxygen species, through energy transfer (singlet oxygen 102) and electron transfer (reduced active oxygen radicals such as superoxide anion radical O2-○ and hydroxyl radical ○OH), was studied by a combination of methods, including biochemical (DNA-cleavage assay in the presence of various scavengers of active oxygen species), physicochemical (EPR radical trapping and near-infrared spectrometry), and chemical methods (nitro blue tetrazolium (NBT) method). Whereas 1O2 was generated effectively by photoexcited C60 in nonpolar solvents such as benzene and benzonitrile, we found that O2-○ and ○OH were produced instead of 1O2 in polar solvents such as water, especially in the presence of a physiological concentration of reductants including NADH. The above results, together with those of a DNA cleavage assay in the presence of various scavengers of specific active oxygen species, indicate that the active oxygen species primarily responsible for photoinduced DNA cleavage by C60 under physiological conditions are reduced species such as O2-○ and ○OH.

Revue / Journal Title

Journal of the American Chemical Society    ISSN  0002-7863   CODEN JACSAT 

Source / Source

2003, vol. 125, no42, pp. 12803-12809 [7 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

American Chemical Society, Washington, DC, ETATS-UNIS  (1879) (Revue)

Mots-clés anglais / English Keywords

EPR spectrometry

;

Near infrared spectrometry

;

Nitroblue tetrazolium test

;

Organic free radical

;

Inorganic free radical

;

Reaction intermediate

;

Fullerene compounds

;

Photosensitizer

;

Near ultraviolet radiation

;

Nucleic acid

;

Oxidative degradation

;

Photochemical reaction

;

Mots-clés français / French Keywords

Spectrométrie RPE

;

Spectrométrie IR proche

;

Test nitrobleu tétrazolium

;

Radical libre organique

;

Radical libre minéral

;

Intermédiaire réaction

;

Fullerène composé

;

Photosensibilisant

;

Rayonnement UV proche

;

Acide nucléique

;

Dégradation oxydante

;

Réaction photochimique

;

Mots-clés espagnols / Spanish Keywords

Espectrometría RPE

;

Espectrometría IR próximo

;

Prueba nitroazul tetrazolio

;

Radical libre orgánico

;

Radical libre inorgánico

;

Intermediario reacción

;

Fotosensibilizante

;

Radiación ultravioleta próxima

;

Acido nucleico

;

Degradación oxidante

;

Reacción fotoquímica

;

Localisation / Location

INIST-CNRS, Cote INIST : 551, 35400011335024.0400

Nº notice refdoc (ud4) : 15214814



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