Titre du document / Document title

IL-18 mRNA expression and IFN-γ induction in bronchoalveolar lavage from Behçet's disease

Auteur(s) / Author(s)

HAMZAOUI A. ^{(1 2)} ; GHRAÏRI H. ^{(1 2)} ; AMMAR J. ^{(1 2)} ; ZEKRI S. ⁽²⁾ ; GUEMIRA F. ⁽³⁾ ; HAMZAOUI K. ⁽²⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Respiratory Diseases, Pneumology Hospital A. Mami, Ariana, TUNISIE
⁽²⁾ Homeostasis and Cell Dysfunction Unit Research, Medicine University of Tunis, Tunis, TUNISIE
⁽³⁾ Biochemical and Oncology Laboratory, Medicine University of Tunis, Tunis, TUNISIE

Résumé / Abstract

Objective. IL-18 expression and functional activity has been identified in several autoimmune and infectious diseases. To clarify the potential role of IL-18 during pulmonary Behçet's disease (BD), we have explored the capacity of IL-18 to induce the expression of IFN-γ. Methods. We studied bronchoalveolar lavage (BAL) from 12 patients with BD, 10 patients with silicosis as the control disease and 10 BAL from healthy subjects. BAL fluid, and BAL fluid cell cultures were investigated for IL-18 estimation by ELISA. Analysis of IL-18 and IFN-γ gene expression was carried out before and after LPS stimulation. Results. BD patients had significantly elevated levels of IL-18 in BAL fluid compared with control disease and healthy subjects. Induction of IFN-y and IL-18 were observed from BD-BAL fluid cells both spontaneously and after LPS stimulation, at higher levels compared to silicosis patients and healthy subjects (HC). Spontaneously only BD BAL cells expressed IL-18 mRNA and IFN-γ mRNA. Forty-eight hours after LPS stimulation IL-18 mRNA and IFN-ymRNA were observed in BD, silicosis and HC cells. Recombinant IL-18 induced IFN-γ production in BD- BAL fluid cells. Conclusion. Administration of IL-18 induced greater IFN-y production in BD-BAL fluid cells, than in normal BAL fluid cells. Our data indicate that IL-18 up-regulation is a feature of BD and suggest that IL-18 and IFN-ymay contribute to the local inflammatory response in BD.

Revue / Journal Title

Clinical and experimental rheumatology   ISSN 0392-856X 

Source / Source

2003, vol. 21, n^o4, pp. S.8-S.14, SUP30 (40 ref.)

Langue / Language

Anglais

Editeur / Publisher

Clinical and Experimental Rheumatology, Pisa, ITALIE  (1983) (Revue)

Mots-clés anglais / English Keywords

Cytokine ; Vascular disease ; Cardiovascular disease ; Systemic disease ; Genital diseases ; Eye disease ; Stomatology ; Skin disease ; Human ; Cellular immunity ; Autoimmunity ; Cell culture ; Messenger RNA ; Gene expression ; Bronchoalveolar lavage ; Th1 lymphocyte ; Gamma interferon ; Interleukin 18 ; Inflammation ; Pathogenesis ; Behçet syndrome ;

Mots-clés français / French Keywords

Cytokine ; Vaisseau sanguin pathologie ; Appareil circulatoire pathologie ; Maladie système ; Appareil génital pathologie ; Oeil pathologie ; Stomatologie ; Peau pathologie ; Homme ; Immunité cellulaire ; Autoimmunité ; Culture cellulaire ; RNA messager ; Expression génique ; Lavage bronchoalvéolaire ; Lymphocyte Th1 ; Interféron gamma ; Interleukine 18 ; Inflammation ; Pathogénie ; Oculomucocutané Behçet syndrome ;

Mots-clés espagnols / Spanish Keywords

Citoquina ; Vaso sanguíneo patología ; Aparato circulatorio patología ; Enfermedad sistémica ; Aparato genital patología ; Ojo patología ; Estomatología ; Piel patología ; Hombre ; Inmunidad celular ; Autoinmunidad ; Cultivo celular ; RNA mensajero ; Expresión genética ; Lavado broncoalveolar ; Linfocito Th1 ; Interferón gamma ; Interleuquina 18 ; Inflamación ; Patogenia ; Behçet síndrome ;

Mots-clés d'auteur / Author Keywords

Behçet's disease ; inflammation ; cytokines ; Th1 ; IL-18 ; IFN-y ;

Localisation / Location

INIST-CNRS, Cote INIST : 20920, 35400011471704.0030