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Titre du document / Document title

Determination of 4-aminophenol impurities in multicomponent analgesic preparations by HPLC with amperometric detection

Auteur(s) / Author(s)

WYSZECKA-KASZUBA E. (1) ; WAROWNA-GRZESKIEWICZ M. (1 2) ; FIJAŁEK Z. (1 2) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Department of Pharmaceutical Chemistry, Drug Institute, National Institute of Public Health, 30/34 Chełmska Str., 00-725 Warsaw, POLOGNE
(2) Department of Drug Analysis, Warsaw Medical University, 1 Banacha Str., 02-097 Warsaw, POLOGNE

Résumé / Abstract

A method for the determination of 4-aminophenol, the main impurity of paracetamol, by high-performance liquid chromatographic (HPLC) method with amperometric detection has been developed. The analysis was performed in an isocratic mode on a reversed phase Luna column 5 μm C-18 (100 x 4.6 mm). A mobile phase (0.05 mol l-1 LiCI solution containing 18% methanol adjusted to pH 4.0 with orthophosphoric acid) was suitable for the separation and determination of 4-APh. Chromatograms were recorded for 250 s by means of an amperometric detector at a potential of +325 mV of the glassy carbon electrode versus the reference electrode Ag/AgCl. The proposed liquid chromatographic method was successfully applied to the analysis of commercially available multicomponent dosage forms. The sensitivity of the detection for 4-aminophenol was I ng ml-1 for substance and 4 ng ml-1 for tablets or capsules. The method developed in this study is sensitive and selective and can be applied for routine studies of pharmaceuticals in the form of tablets or capsules.

Revue / Journal Title

Journal of pharmaceutical and biomedical analysis    ISSN  0731-7085   CODEN JPBADA 

Source / Source

Congrès
Drug Analysis 2002, Bruges , BELGIQUE (21/04/2002)
2003, vol. 32, no 4-5 (546 p.)  [Document : 6 p.] (11 ref.), [Notes: Joint Meeting of the 7th International Symposium on Drug Analysis and of the 13th International Symposium on Pharmaceutical and Biomedical Analysis], pp. 1081-1086 [6 page(s) (article)]

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1983) (Revue)

Mots-clés anglais / English Keywords

Derived product

;

Phenol

;

Amperometry

;

Analysis method

;

HPLC chromatography

;

Reversed phase chromatography

;

Analgesic

;

Paracetamol

;

Quantitative analysis

;

Mots-clés français / French Keywords

Produit dérivé

;

Phénol

;

Ampérométrie

;

Méthode analyse

;

Chromatographie HPLC

;

Chromatographie phase inverse

;

Analgésique

;

Paracétamol

;

Analyse quantitative

;

Mots-clés espagnols / Spanish Keywords

Producto derivado

;

Fenol

;

Amperometría

;

Método análisis

;

Cromatografía HPLC

;

Cromatografía fase inversa

;

Analgésico

;

Paracetamol

;

Análisis cuantitativo

;

Mots-clés d'auteur / Author Keywords

4-Aminophenol; Paracetamol; HPLC method with amperometric detection

;

determination; Pharmaceutical formulations

;

Localisation / Location

INIST-CNRS, Cote INIST : 19962, 35400011228559.0510

Nº notice refdoc (ud4) : 15055187



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