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Titre du document / Document title

Stimulation of furanochromone accumulation in callus cultures of Ammi visnaga L. by addition of elicitors

Auteur(s) / Author(s)

KROLICKA A. (1) ; STANISZEWSKA I. (2) ; MALINSKI E. (2) ; SZAFRANEK J. (2) ; ŁOJKOWSKA E. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Faculty of Biotechnology, University of Gdansk and Medical University of Gdansk, POLOGNE
(2) Faculty of Chemistry, University of Gdansk, POLOGNE

Résumé / Abstract

In order to check the possibility of producing secondary metabolites, in vitro cultures of A. visnaga callus were established. The best growth of A. visnaga callus was obtained on Murashige and Skoog medium (MS) containing 6-benzyladenine (BA) and α-naphtaleneacetic acid (NAA). The study was concentrated on the induction of production of secondary metabolites by exposing callus to abiotic elicitors: benzo(1,2,3)-thiadiazole-7-carbothionic acid S-methyl ester (BION®) and a suspension of silica (SiO2) and biotic elicitors: autoclaved lysates of Enterobacter sakazaki and scleroglucan. GC analysis indicated that not-elicited callus of A. visnaga grown in darkness accumulated 2 times more visnagin than the one which was grown under a 16-h photoperiod. The highest accumulation of visnagin was observed in the callus culture elicited with scleroglucan or BION®. Scleroglucan induced also the accumulation of khellin in A. visnaga callus. The presented work shows that biosynthesis of pharmacologically important secondary metabolites in A. visnaga cultures could be stimulated by application of elicitors.

Revue / Journal Title

Pharmazie    ISSN  0031-7144   CODEN PHARAT 

Source / Source

2003, vol. 58, no8, pp. 590-592 [3 page(s) (article)] (23 ref.)

Langue / Language

Anglais
Revue : Allemand

Editeur / Publisher

Govi, Eschborn, ALLEMAGNE  (1946) (Revue)

Mots-clés anglais / English Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Antispasmodic agent

;

Vasodilator agent

;

Aromatic compound

;

Tricyclic compound

;

Oxygen heterocycle

;

Khellin

;

Elicitor

;

Umbelliferae

;

Medicinal plant

;

Plant tissue

;

Tissue culture

;

Culture medium

;

Production

;

Optimization

;

Mots-clés français / French Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Ammi visnaga

;

Furobenzopyrane

;

Furo[3,2-g][1]benzopyran-5-one dérivé

;

Visnagine

;

1,2,3-Benzothiadiazole-7-carbothioïque acide ester S-méthyle

;

Scléroglucane

;

Spasmolytique

;

Vasodilatateur

;

Composé aromatique

;

Composé tricyclique

;

Hétérocycle oxygène

;

Khelline

;

Eliciteur

;

Umbelliferae

;

Plante médicinale

;

Tissu végétal

;

Culture tissu

;

Milieu culture

;

Production

;

Optimisation

;

Mots-clés espagnols / Spanish Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Espasmolítico

;

Vasodilatador

;

Compuesto aromático

;

Compuesto tricíclico

;

Heterociclo oxígeno

;

Kelina

;

Elicitador

;

Umbelliferae

;

Planta medicinal

;

Tejido vegetal

;

Cultivo tejido

;

Medio cultivo

;

Producción

;

Optimización

;

Localisation / Location

INIST-CNRS, Cote INIST : 3582, 35400011261691.0160

Nº notice refdoc (ud4) : 15029055



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