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Titre du document / Document title

Dentin matrix protein 1 is expressed in human lung cancer

Auteur(s) / Author(s)

CHAPLET M. (1) ; DE LEVAL L. (2) ; WALTREGNY D. (1) ; DETRY C. (1) ; FORNACIARI G. (3) ; BEVILACQUA G. (3) ; FISHER L. W. (4) ; CASTRONOVO V. (1) ; BELLAHCENE A. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Metastasis Research Laboratory, University of Liège, Liège, BELGIQUE
(2) Department of Pathology, Center of Experimental Cancer Research, University of Liège, Liège, BELGIQUE
(3) Department of Pathology, University of Pisa, Pisa, ITALIE
(4) Craniofacial and Skeletal Diseases Branch, NIDCR, National Institutes of Health, H.H.S., Bethesda, Maryland, ETATS-UNIS

Résumé / Abstract

We have previously shown that breast and prostate cancers express bone matrix proteins. DMP1 expression was evaluated in 59 human lung cancer samples at the protein and mRNA levels. It was detectable in 80% of the cases, suggesting a potential role for DMP1 in tumor progression and bone metastasis. Introduction: Previously, we and others have shown that bone extracellular matrix proteins such as bone sialoprotein (BSP) and osteopontin (OPN) are expressed in various types of cancer that are characterized by a high affinity for bone including breast, prostate, and lung adenocarcinoma. Based on biochemical and genetic features, BSP, OPN, dentin matrix protein 1 (DMP1), and dentin sialophosphoprotein (DSPP) have been recently classified in a unique family named SIBLING (small integrin-binding ligand, N-linked glycoprotein). Therefore, we investigated whether DMP1 could also be detected in osteotropic cancers. Materials and Methods: We first used a cancer array for evaluating the relative abundance of DMPI transcript in a broad spectrum of human cancer tissues. This screening showed that DMP1 was strongly detectable in lung tumors compared with normal corresponding tissue. In a second step, we used an immunophosphatase technique and a specific polyclonal antibody directed against DMP1 to examine the expression of DMPI in 59 human non-small cell lung cancer samples, including 29 squamous carcinoma, 20 adenocarcinoma, and 10 bronchioloalveolar carcinoma. Student's t-test was used to determine the statistical significance of immunostaining scores between the lung cancer histological groups studied and between cancer and normal lung tissues. Results: Our results show that DMP1 is detectable in 90% of the adenocarcinoma and squamous carcinoma analyzed while 8 of 10 bronchioloalveolar specimens were negative. DMPI immunostaining intensity and extent scores were significantly higher in adenocarcinoma (p = 0.0004) and squamous carcinoma (p < 0.0001) samples compared with adjacent normal lung tissue. In situ hybridization experiments confirmed that DMP1 mRNA is localized in lung cancer cells. Conclusion: In this study, we show that a third SIBLING protein is ectopically expressed in lung cancer. The role of DMP1 in lung cancer is largely unknown. Further studies are required to determine the implication of this protein, next to its sisters SIBLING proteins, in tumor progression and bone metastasis development.

Revue / Journal Title

Journal of bone and mineral research    ISSN  0884-0431   CODEN JBMREJ 

Source / Source

2003, vol. 18, no8, pp. 1506-1512 [7 page(s) (article)] (21 ref.)

Langue / Language

Anglais

Editeur / Publisher

Wiley, Hoboken, NJ, ETATS-UNIS  (1986) (Revue)

Mots-clés anglais / English Keywords

Malignant tumor

;

Bronchus disease

;

Lung disease

;

Respiratory disease

;

Osteoarticular system

;

Human

;

Carcinogenesis

;

Pathogenesis

;

Diseases of the osteoarticular system

;

Metastasis

;

Bronchopulmonary

;

Tumor

;

Bone

;

Extracellular matrix

;

Dentin

;

Protein

;

Gene expression

;

Mots-clés français / French Keywords

Tumeur maligne

;

Bronche pathologie

;

Poumon pathologie

;

Appareil respiratoire pathologie

;

Système ostéoarticulaire

;

Homme

;

Carcinogenèse

;

Pathogénie

;

Système ostéoarticulaire pathologie

;

Métastase

;

Bronchopulmonaire

;

Tumeur

;

Os

;

Matrice extracellulaire

;

Dentine

;

Protéine

;

Expression génique

;

Mots-clés espagnols / Spanish Keywords

Tumor maligno

;

Bronquio patología

;

Pulmón patología

;

Aparato respiratorio patología

;

Sistema osteoarticular

;

Hombre

;

Carcinogénesis

;

Patogenia

;

Sistema osteoarticular patología

;

Metástasis

;

Broncopulmonar

;

Tumor

;

Hueso

;

Matriz extracelular

;

Dentina

;

Proteína

;

Expresión genética

;

Mots-clés d'auteur / Author Keywords

dentin matrix protein 1

;

bone metastasis

;

lung cancer

;

Localisation / Location

INIST-CNRS, Cote INIST : 21114, 35400011256550.0140

Nº notice refdoc (ud4) : 15005341



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