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Titre du document / Document title

Chromatographic separation of phenolic compounds from rapeseed by a Sephadex LH-20 column with ethanol as the mobile phase

Auteur(s) / Author(s)

AMAROWICZ R. (1) ; KOLODZIEJCZYK P. P. (2) ; PEGG R. B. (3) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Division of Food Science, Institute of Animal Reproduction and Food Research, Polish Academy of Sciences, Olsztyn, POLOGNE
(2) Olds College Centre for Innovation, Olds, CANADA
(3) Saskatchewan Food Product Innovation Program, Department of Applied Microbiology and Food Science, University of Saskatchewan, Saskatoon, CANADA

Résumé / Abstract

Phenolic compounds from rapeseed were chromatographed using an open column filled with Sephadex LH-20 as the stationary phase and ethanol as the mobile phase. Typically, methanol has been used as the eluent of choice in previous studies, but it was hypothesized that ethanol's lesser polarity would provide better separation of the rapeseed phenolics. In this work, three fractions (I-III) containing phenolic compounds were collected; their characteristics were evaluated qualitatively and quantitatively by UV spectrophotometry, a colorimetric assay, and capillary electrophoresis (CE). Although fraction I possessed the greatest relative proportion of the crude extract (62.2%), fraction III contained the richest source of rapeseed phenolics (176 mg transsinapic acid equivalents g-1). Capillary electrophoresis identified sinapine as the dominant phenolic compound of fraction I and indicated that a number of phenolic compounds were present in fractions II and III. Although free and esterified derivatives of phenolic acids were constituents of fraction II, the electropherogram revealed that sinapic acid was the chief phenolic compound present, based on retention time mapping. Application of ethanol as the eluent, rather than methanol, gave a better baseline separation of the phenolic constituents from rapeseed.

Revue / Journal Title

Journal of liquid chromatography & related technologies    ISSN  1082-6076   CODEN JLCTFC 

Source / Source

2003, vol. 26, no13, pp. 2157-2165 [9 page(s) (article)] (20 ref.)

Langue / Language

Anglais

Editeur / Publisher

Taylor & Francis, Colchester, ROYAUME-UNI  (1996) (Revue)

Mots-clés anglais / English Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Cruciferae

;

Ultraviolet detector

;

Capillary electrophoresis

;

Zone electrophoresis

;

Gel permeation chromatography

;

Column chromatography

;

Pharmacognosy

;

Phenolic acid

;

Antioxidant

;

Rapeseed

;

Brassica napus

;

Oilseed

;

Medicinal plant

;

Plant origin

;

Qualitative analysis

;

Quantitative analysis

;

Chemical analysis

;

Mots-clés français / French Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Cruciferae

;

Détecteur UV

;

Electrophorèse capillaire

;

Electrophorèse zone

;

Chromatographie gel

;

Chromatographie colonne

;

Pharmacognosie

;

Acide phénolique

;

Antioxydant

;

Graine colza

;

Brassica napus

;

Graine oléagineuse

;

Plante médicinale

;

Origine végétale

;

Analyse qualitative

;

Analyse quantitative

;

Analyse chimique

;

Mots-clés espagnols / Spanish Keywords

Spermatophyta

;

Angiospermae

;

Dicotyledones

;

Cruciferae

;

Detector UV

;

Electroforesis capilar

;

Electroforesis zonal

;

Cromatografía gel

;

Cromatografía columna

;

Farmacognosia

;

Acido fenólico

;

Antioxidante

;

Semilla colza

;

Brassica napus

;

Semilla oleaginosa

;

Planta medicinal

;

Origen vegetal

;

Análisis cualitativo

;

Análisis cuantitativo

;

Análisis químico

;

Localisation / Location

INIST-CNRS, Cote INIST : 18068, 35400011948545.0100

Nº notice refdoc (ud4) : 14995946



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