Titre du document / Document title

Application of metal nanoparticles in confocal laser scanning microscopy: improved resolution by optical field enhancement

Auteur(s) / Author(s)

ALSCHINGER M. ⁽¹⁾ ; MANIAK M. ^{(2 3)} ; STIETZ F. ⁽¹⁾ ; VARTANYAN T. ⁽¹⁾ ; TRÄGER F. ^{(1 3)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Experimentalphysik I, Universität Kassel, Heinrich-Plett-Str. 40, 34132 Kassel, ALLEMAGNE
⁽²⁾ Zellbiologie, Universität Kassel, Heinrich-Plett-Str. 40, 34132 Kassel, ALLEMAGNE
⁽³⁾ Center for Interdisciplinary Nanostructure Science and Technology - CINSAT, Universität Kassel, Heinrich-Plett-Str. 40, 34132 Kassel, ALLEMAGNE

Résumé / Abstract

We describe experiments in which the field enhancement by metal nanoparticles that accompanies surface plasmon excitation has been exploited in confocal laser scanning microscopy. The objective was to make use of the rapid decay of the enhanced light field with distance from the nanoparticles to increase the longitudinal resolution, by confining the emission of fluorescence light to the immediate vicinity of the contact area between the substrate and the objects under study. We demonstrate that the increased longitudinal resolution is accompanied by a better lateral resolution if the imaged objects have a curved surface.

Revue / Journal Title

Applied physics. B, Lasers and optics    ISSN  0946-2171 

Source / Source

2003, vol. 76, n^o7, pp. 771-774 [4 page(s) (article)] (20 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Heidelberg, ALLEMAGNE  (1994) (Revue)

Mots-clés anglais / English Keywords

Transition elements

;

Silver

;

Biological tissues

;

Nanoparticles

;

Spatial resolution

;

Surface plasmons

;

Experimental study

;

Scanning microscope

;

Laser microscope

;

Confocal microscopy

;

Mots-clés français / French Keywords

Métal transition

;

Ag

;

Argent

;

Tissu biologique

;

Nanoparticule

;

Résolution spatiale

;

Plasmon surface

;

Etude expérimentale

;

Microscope balayage

;

Microscope laser

;

Microscopie confocale

;

Mots-clés espagnols / Spanish Keywords

Microscopio barrido

;

Microscopio láser

;

Microscopía confocal

;

Localisation / Location

INIST-CNRS, Cote INIST : 16194 B, 35400011136406.0100