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Titre du document / Document title

Characteristics of the epidermis and stratum corneum of hairless mice with experimentally induced diabetes mellitus

Auteur(s) / Author(s)

SAKAI Shingo (1) ; ENDO Yoko (1) ; OZAWA Naoko (1) ; SUGAWARA Tomoko (1) ; KUSAKA Ayumi (1) ; SAYO Tetsuya (1) ; TAGAMI Hachiro (2) ; INOUE Shintaro (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Basic Research Laboratory, Kanebo Ltd, Kanagawa 250-0002, JAPON
(2) Department of Dermatology, Tohoku University School of Medicine, Sendai, JAPON

Résumé / Abstract

Diabetes mellitus induces many pathophysiologic changes in the skin. Even so, dermatologists still lack an animal model of diabetes that enables the direct evaluation of the various functional properties of the skin. Our group induced two types of an experimental type 1 diabetes model in hairless mice by administering either streptozotocin or alloxan, in order to examine the properties of the stratum corneum and epidermis of these animals. The plasma glucose concentrations of the mice at 3 wk after their i.v. injection were significantly higher than those of control mice (streptozotocin, 3.2-fold; alloxan, 3.7-fold). The stratum corneum water content was significantly reduced in both types of diabetic mice, whereas the transepidermal water loss remained unchanged. The amino acid content with normal epidermal profilaggrin processing was either normal or elevated in the stratum corneum of the streptozotocin-treated mice. In contrast, the stratum corneum triglyceride content in the streptozotocin-treated mice was significantly lower than the control level, even though the levels of ceramides, cholesterols, and fatty acids in the stratum corneum were all higher than the control levels. The streptozotocin-treated group also exhibited decreases in basal cell proliferation and epidermal DNA content linked with an increase in the number of corneocyte layers in the stratum corneum, suggesting that the rates of epidermal and stratum corneum turnover were slower in the streptozotocin-treated animals than in the normal controls. In contrast, there were no remarkable changes in any of the epidermal differentiation marker proteins examined. This finding in diabetic mice, namely, reduction in both the epidermal proliferation and stratum corneum water content without any accompanying impairment in the stratum corneum barrier function, is similar to that found in aged human skin. Our new animal model of diabetes will be useful for the study of diabetic dermopathy as well as the mechanisms of stratum corneum moisturization.

Revue / Journal Title

Journal of investigative dermatology    ISSN  0022-202X   CODEN JIDEAE 

Source / Source

2003, vol. 120, no1, pp. 79-85 [7 page(s) (article)] (58 ref.)

Langue / Language

Anglais

Editeur / Publisher

Nature Publishing Group, New York, NY, ETATS-UNIS  (1938) (Revue)

Mots-clés anglais / English Keywords

Endocrinopathy

;

Vertebrata

;

Mammalia

;

Rodentia

;

Skin

;

Mouse

;

Animal

;

Stratum corneum

;

Diabetes mellitus

;

Epidermis

;

Mots-clés français / French Keywords

Endocrinopathie

;

Vertebrata

;

Mammalia

;

Rodentia

;

Peau

;

Souris

;

Animal

;

Couche cornée

;

Diabète

;

Epiderme

;

Mots-clés espagnols / Spanish Keywords

Endocrinopatía

;

Vertebrata

;

Mammalia

;

Rodentia

;

Piel

;

Ratón

;

Animal

;

Estrato córneo

;

Diabetes

;

Epidermis

;

Localisation / Location

INIST-CNRS, Cote INIST : 3194, 35400011856847.0120

Nº notice refdoc (ud4) : 14950742



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