Titre du document / Document title

GISH confirmation of somatic hybrids between Solanum melongena and S. torvum: assessment of resistance to both fungal and bacterial wilts

Auteur(s) / Author(s)

COLLONNIER Cécile ⁽¹⁾ ; FOCK Isabelle ⁽²⁾ ; MARISKA Ika ⁽³⁾ ; SERVAES Aline ⁽¹⁾ ; VEDEL Fernand ⁽¹⁾ ; SILJAK-YAKOVLEV Sonja ⁽¹⁾ ; SOUVANNAVONG Vongthip ⁽⁴⁾ ; SIHACHAKR Darasinh ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Laboratoire d'Écologie, Systématique et Évolution, Université Paris Sud, UPS/CNRS (UMR 8079), Bât. 360, 91405 Orsay, FRANCE
⁽²⁾ Laboratoire de Biologie et Physiologie Végétales, Génétique Moléculaire et Évolutive, Université de la Réunion, 15, avenue René-Cassin, BP 7151, 97715 Saint-Denis, FRANCE
⁽³⁾ Research Institute for Food Crops Biotechnology, JL Tentara Pelajar No. 3A, Bogor 16610, INDONESIE
⁽⁴⁾ Groupe Endotoxines, Université Paris Sud, UPS/CNRS (UMR 8619), Bât 430, 91405 Orsay, FRANCE

Résumé / Abstract

Interspecific somatic hybrids between Solanum melongena L. (2n = 2x = 24) and two accessions of Solanum torvum Sw. (2n = 2x = 24) were produced in view of transferring resistance to two soil-born pathogens, Ralstonia solanacearum and Verticillium dahliae, from the wild species into the cultivated eggplant. All somatic hybrids were phenotypically homogenous and intermediate between the parents. Their hybrid nature was confirmed by analysis of isozymes and RAPDs. They showed reduced pollen viability, and all but one possessed the chloroplasts from either one or the other parent. As S. melongena and S. torvum chromosomes were morphologically indistinguishable, genomic in situ hybridisation (GISH) was applied to recognise the chromosomes from each parent in the hybrids. As expected, the selected tetraploid plants contained one complete set of chromosomes from each fusion partner. On spread preparations, the two parental genomes were not spatially separated at any time of the cell cycle. Translocation or recombinant chromosomes could not be demonstrated in the mitotic metaphase. Tests for resistance performed in vitro by using suspensions of two strains of R. solanacearum (race 1 and 3) and filtrate of culture medium of one strain of V. dahliae, revealed that S. melongena was susceptible, whereas both accessions of S. torvum had high levels of resistance. Except for two hybrid clones, which were found susceptible to race 3, as was S. melongena, all somatic hybrids tested showed good levels of bacterial and fungal resistance, either intermediate or as high as that of the wild parent.

Revue / Journal Title

Plant physiology and biochemistry   ISSN 0981-9428   CODEN PPBIEX 

Source / Source

2003, vol. 41, n^o5, pp. 459-470 [12 page(s) (article)] (36 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Paris, FRANCE  (1987) (Revue)

Mots-clés anglais / English Keywords

Vegetable crop ; Spermatophyta ; Angiospermae ; Dicotyledones ; Solanaceae ; Genomic in situ hybridisation ; Solanum melongena ; Microorganism resistance ; Somatic hybrid ; Interspecific hybridization ;

Mots-clés français / French Keywords

Plante légumière ; Spermatophyta ; Angiospermae ; Dicotyledones ; Solanaceae ; Hybridation génomique in situ ; Solanum torvum ; Solanum melongena ; Résistance microorganisme ; Hybride somatique ; Hybridation interspécifique ;

Mots-clés espagnols / Spanish Keywords

Hortalizas (plantas) ; Spermatophyta ; Angiospermae ; Dicotyledones ; Solanaceae ; Solanum melongena ; Resistencia microorganismo ; Híbrido somático ; Hibridación interespecífica ;

Mots-clés d'auteur / Author Keywords

GISH ; In vitro tests ; Protoplast electrofusion ; Ralstonia solanacearum ; Solanum melongena ; Solanum torvum ; Verticillium dahliae ;

Localisation / Location

INIST-CNRS, Cote INIST : 10570, 35400011838217.0090