Titre du document / Document title

Ocular hypotensive effects of cholinergic and adrenergic drugs may be influenced by prostaglandins E2 in the human and rabbit eye

Auteur(s) / Author(s)

KAPLAN-MESSAS A. ⁽¹⁾ ; NAVEH N. ⁽²⁾ ; AVNI I. ⁽¹⁾ ; MARSHALL J. ⁽³⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Assaf Harofe Medical Center, Tsrifin, ISRAEL
⁽²⁾ Goldshleger Eye Research Institute, Tel Aviv University, Tel Aviv, ISRAEL
⁽³⁾ Raynes Institute, Saint Thomas Hospital, London, ROYAUME-UNI

Résumé / Abstract

PURPOSE. Increased PGE2 production by the iris and ciliary body regulate intraocular pressure (IOP) in vivo. Various cholinergic and adrenergic compounds are traditionally used as antiglaucoma drugs, and their effect on IOP reduction is antagonised by cyclooxygenase inhibitors, indicating a role for eicosanoids in their hypotensive activity. One of the most potent antiglaucoma drugs, PG2 alpha (Latanoprost), reduces IOP by increasing uveoscle-ral outflow and also increases PGE2 production by the iris and ciliary body in vivo. We investigated whether cholinergic and adrenergic antiglaucoma drugs induce the production of prostaglandin E2 (PGE2) in vitro by: 1) the iris-ciliary body (ICB) of rabbits and, 2) irises of glaucoma patients. METHODS. Pilocarpine 2%, epinephrine 1% and echothiophate iodide 0.125% were applied topically to both eyes of Albino rabbits. Control groups were treated with the corresponding vehicles, or untreated completely. Human iris specimens were obtained from nine untreated cataract eyes, and five eyes under antiglaucoma medication undergoing surgery. PGE2 were determined by a radioimmunoassay. RESULTS. PGE2 production by the ICB of treated rabbits in vitro was twice that of vehicle-treated or untreated rabbit eyes (p< 0.001, for either group). In vitro PGE2 production by treated glaucoma patients' irises was three times higher (p<0.001) than in cataract control patients. CONCLUSIONS. The study found an increase in in vitro production of PGE2 by the irises of eyes treated with cholinergic and adrenergic antiglaucoma medications. This suggests a role for endogenous PG production in the hypotensive effect of both classes of drug.

Revue / Journal Title

European Journal of Ophthalmology   ISSN 1120-6721 

Source / Source

2003, vol. 13, n^o1, pp. 18-23 [6 page(s) (article)] (21 ref.)

Langue / Language

Anglais

Editeur / Publisher

Wichtig, Milano, ITALIE  (1991) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Lagomorpha ; Experimental study ; In vitro ; Mechanism of action ; Antiglaucomatous agent ; Prostaglandin E2 ; Animal ; Rabbit ; Human ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Lagomorpha ; Etude expérimentale ; In vitro ; Mécanisme action ; Antiglaucomateux ; Prostaglandine E2 ; Animal ; Lapin ; Homme ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Lagomorpha ; Estudio experimental ; In vitro ; Mecanismo acción ; Antiglaucomatoso ; Prostaglandina E2 ; Animal ; Conejo ; Hombre ;

Mots-clés d'auteur / Author Keywords

Prostaglandin E2 ; Iris-ciliary body ; Rabbit ; Cholinergic ; Adrenergic ;

Localisation / Location

INIST-CNRS, Cote INIST : 22516, 35400011960557.0030