Titre du document / Document title

Use of the mannitol pathway in fructose fermentation of Oenococcus oeni due to limiting redox regeneration capacity of the ethanol pathway

Auteur(s) / Author(s)

RICHTER Hanno ⁽¹⁾ ; HAMANN Inka ⁽¹⁾ ; UNDEN Gottfried ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Institut für Mikrobiologie und Weinforschung, Johannes Gutenberg-Universität Mainz, Becherweg 15, 55099 Mainz, ALLEMAGNE

Résumé / Abstract

The heterolactic bacterium Oenococcus oeni ferments fructose by a mixed heterolactic/mannitol fermentation. For heterolactic fermentation of fructose, the phosphoketolase pathway is used. The excess NAD(P)H from the phosphoketolase pathway is reoxidized by fructose (yielding mannitol). It is shown here that, under conditions of C-limitation or decreased growth rates, fructose can be fermented by heterolactic fermentation yielding nearly stoichiometric amounts of lactate, ethanol and CO₂. Quantitative evaluation of NAD(P)H-producing (phosphoketolase pathway) and -reoxidizing (ethanol, mannitol and erythritol pathways) reactions demonstrated that at high growth rates or in batch cultures the ethanol pathway does not have sufficient capacity for NAD(P)H reoxidation, requiring additional use of the mannitol pathway to maintain the growth rate. In addition, insufficient capacities to reoxidize NAD(P)H causes inhibition of growth, whereas increased NAD(P)H reoxidation by electron acceptors such as pyruvate increases the growth rate.

Revue / Journal Title

Archives of microbiology   ISSN 0302-8933   CODEN AMICCW 

Source / Source

2003, vol. 179, n^o4, pp. 227-233 [7 page(s) (article)] (10 ref.)

Langue / Language

Anglais

Editeur / Publisher

Springer, Berlin, ALLEMAGNE  (1974) (Revue)

Mots-clés anglais / English Keywords

Enzyme ; Lyases ; Carbon-carbon lyases ; Aldehyde-lyases ; Bacteria ; Diagnostic agent ; Diuretic ; Mannitol ; Batchwise ; Quantitative analysis ; Growth rate ; Phosphoketolase ; Ethanol ; Regeneration ; Fermentation ; Fructose ; Oenococcus oeni ;

Mots-clés français / French Keywords

Enzyme ; Lyases ; Carbon-carbon lyases ; Aldehyde-lyases ; Bactérie ; Produit diagnostic ; Diurétique ; Mannitol ; En discontinu ; Analyse quantitative ; Taux croissance ; Phosphoketolase ; Ethanol ; Régénération ; Fermentation ; Fructose ; Oenococcus oeni ;

Mots-clés espagnols / Spanish Keywords

Enzima ; Lyases ; Carbon-carbon lyases ; Aldehyde-lyases ; Bacteria ; Producto diagnóstico ; Diurético ; Manitol ; En discontinuo ; Análisis cuantitativo ; Tasa crecimiento ; Phosphoketolase ; Etanol ; Regeneración ; Fermentación ; Fructosa ; Oenococcus oeni ;

Mots-clés d'auteur / Author Keywords

Oenococcus oeni ; Heterolactic fermentation ; Fructose ; Phosphoketolase pathway ; NAD(P)H reoxidation ; Mannitol ;

Localisation / Location

INIST-CNRS, Cote INIST : 856, 35400011804466.0010