Titre du document / Document title

The role of hypothalamic histamine in leptin-induced suppression of short-term food intake in fasted rats

Auteur(s) / Author(s)

TOFTEGAARD C. L. ⁽¹⁾ ; KNIGGE U. ^{(1 2)} ; KJAER A. ^{(1 3)} ; WARBERG J. ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Medical Physiology, The Panum Institute Building 123, 3, University of Copenhagen, Blegdamsvej 3, Copenhagen, 2200, DANEMARK
⁽²⁾ Department of Surgery C, Rigshospitalet, Blegdamsvej 9, Copenhagen 2100, DANEMARK
⁽³⁾ Departments of Clinical Physiology and Nuclear Medicine, Rigshospitalet, Blegdamsvej 9, Copenhagen 2100, DANEMARK

Résumé / Abstract

Objective : Leptin suppresses food intake; however, the precise mechanism is not fully understood. Histamine (HA), which acts as a neurotransmitter in the central nervous system, has also been shown to be involved in feeding and exerts an inhibitory effect through activation of H_i receptors. Therefore, we studied the possible role of HA in short-term leptin-induced suppression of food intake. Methods'. We studied the 6-h feeding response of overnight-fasted adult (200 g) male Wistar rats to leptin and the HA synthesis inhibitor α-fluoromethylhistidine (a-FMH). Levels of transcription for neuropeptide Y (NPY) and corticotropin-releasing hormone (CRH), as well as hypothalamic content of HA and the HA metabolite telemethyl-HA were investigated. Results: Central administration of leptin (3, 5 and 10 μg at 09:00 h) in fasted rats caused a decrease in food intake. In contrast, central administration of α-FMH (11, 22 and 112 μg at 09:00 h) increased food intake. Prior administration of α-FMH prevented the leptin-induced decrease in food intake. Leptin decreased hypothalamic histamine content, while increasing the ratio between telemethyl-HA and HA, indicating that leptin reduces HA metabolism. Finally, α-FMH suppressed basal and leptin-induced CRH expression while stimulating NPY expression in fasted rats. Conclusion: Histamine is involved in leptin-induced inhibition of food intake. The role of histamine may be mediating, i.e. leptin may directly activate and/or change the metabolism of the histaminergic system. Alternatively, the histaminergic system may be involved in a permissive manner.

Revue / Journal Title

Regulatory peptides   ISSN 0167-0115   CODEN REPPDY 

Source / Source

2003, vol. 111, n^o1-3, pp. 83-90 [8 page(s) (article)] (59 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1980) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Rodentia ; Hypothalamic hormone ; Hormone releasing factor ; Central nervous system ; Brain (vertebrata) ; Food intake ; Animal ; Rat ; Short term ; Feeding behavior ; Leptin ; Histamine ; Hypothalamus ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Rodentia ; Hormone hypothalamique ; Facteur libération hormonale ; Système nerveux central ; Encéphale ; Consommation alimentaire ; Animal ; Rat ; Court terme ; Comportement alimentaire ; Leptine ; Histamine ; Hypothalamus ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Rodentia ; Hormona hipotalámica ; Factor liberación hormonal ; Sistema nervioso central ; Encéfalo ; Consumo alimenticio ; Animal ; Rata ; Corto plazo ; Conducta alimenticia ; Leptina ; Histamina ; Hipotálamo ;

Mots-clés d'auteur / Author Keywords

Histamine ; Leptin ; CRF ; NPY ; Food intake ; CNS ;

Localisation / Location

INIST-CNRS, Cote INIST : 18854, 35400010988054.0110