Titre du document / Document title

Spectrophotometric and chromatographic determination of rabeprazole in presence of its degradation products

Auteur(s) / Author(s)

EL-GINDY Alaa ⁽¹⁾ ; EL-YAZBY Fawzy ⁽²⁾ ; MAHER Moustafa M. ⁽³⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Suez Canal University, Ismuiliu 41522, EGYPTE
⁽²⁾ Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Alexandria University, Alexandria, EGYPTE
⁽³⁾ Pharaonia Pharmaceuticals, New Borg El-Arab City, Alexandria, EGYPTE

Résumé / Abstract

Three methods were presented for the determination of rabeprazole (RA) in presence of its degradation products. The first method was based on high performance liquid chromatographic (HPLC) separation of RA from its degradation products on a reversed phase, ODS column using a mobile phase of methanol - water (70:30, v/v) and UV detection at 284 nm. The second method was based on HPTLC separation followed by densitometric measurement of the spots at 284 nm. The separation was carried out on Merck HPTLC sheets of silica gel 60 F 254, using acetone-toluene-methanol (9:9:0.6 v/v) as mobile phase. The third method depends on first derivative of the ratio spectra (¹DD) by measurement of the amplitudes at 310.2 nm. Moreover, the proposed HPLC method was utilized to investigate the kinetics of the oxidative and photo degradation processes. The pH-rate profile of degradation of RA in Britton-Robinson buffer solutions within the pH range 3-11 was studied. In addition, the activation energy of RA degradation was calculated in Britton-Robinson buffer solution pH 7.

Revue / Journal Title

Journal of pharmaceutical and biomedical analysis   ISSN 0731-7085   CODEN JPBADA 

Source / Source

2003, vol. 31, n^o2, pp. 229-242 [14 page(s) (article)] (15 ref.)

Langue / Language

Anglais

Editeur / Publisher

Elsevier, Amsterdam, PAYS-BAS  (1983) (Revue)

Mots-clés anglais / English Keywords

Enzyme ; Hydrolases ; Benzimidazole derivatives ; Proton pump inhibitor ; Enzyme inhibitor ; H⁺/K⁺-exchanging ATPase ; Comparative study ; High performance ; Thin layer chromatography ; Degradation product ; Kinetics ; Densitometry ; Ultraviolet detector ; Spectrophotometry ; HPLC chromatography ; Reversed phase chromatography ; Analysis method ; Quantitative analysis ; Rabeprazole ;

Mots-clés français / French Keywords

Enzyme ; Hydrolases ; Benzimidazole dérivé ; Inhibiteur pompe proton ; Inhibiteur enzyme ; H⁺/K⁺-exchanging ATPase ; Etude comparative ; Haute performance ; Chromatographie couche mince ; Produit dégradation ; Cinétique ; Densitométrie ; Détecteur UV ; Spectrophotométrie ; Chromatographie HPLC ; Chromatographie phase inverse ; Méthode analyse ; Analyse quantitative ; Rabéprazole ;

Mots-clés espagnols / Spanish Keywords

Enzima ; Hydrolases ; Benzimidazol derivado ; Inhibidor bomba protón ; Inhibidor enzima ; H⁺/K⁺-exchanging ATPase ; Estudio comparativo ; Alto rendimiento ; Cromatografía capa fina ; Producto degradación ; Cinética ; Densitometría ; Detector UV ; Espectrofotometría ; Cromatografía HPLC ; Cromatografía fase inversa ; Método análisis ; Análisis cuantitativo ; Rabeprazol ;

Mots-clés d'auteur / Author Keywords

Rabeprazole ; HPLC ; HPTLC-densitometry ; First derivative of the ratio spectra ; Kinetics of degradation ; pH-rate profile ; Arrhenius plot ;

Localisation / Location

INIST-CNRS, Cote INIST : 19962, 35400011090421.0020