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Titre du document / Document title

Positioning of salt gradients in ion-exchange SMB

Auteur(s) / Author(s)

HOUWING Joukje (1) ; JENSEN Thomas B. (1) ; VAN HATEREN Stef H. (1) ; BILLIET Hugo A. H. (1) ; VAN DER WIELEN Luuk A. M. (1) ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

(1) Kluyver Laboratory for Biotechnology, Delft University of Technology, 2628 BC Delft, PAYS-BAS

Résumé / Abstract

Salt gradients can be used to improve the efficiency of ion-exchange separations in simulated moving-bed systems. The gradient, formed by the use of feed and desorbent solutions of different salt concentrations, introduces regions of increased and decreased affinity of for example, proteins for the matrix. Several gradient shapes can be formed, depending on the flow-rate ratios and salt concentrations used. Only some of these effectively increase throughput or decrease desorbent consumption. Correct gradient positioning is essential, but not trivial, because salt is adsorbed in the resin. A procedure developed selects the flow-rate ratios that allow correct positioning of gradients based on wave theory and incorporates the nonlinear Donnan isotherm of salt on ion-exchange resins. Predictions are verified by experiments combined with a mathematical equilibrium stage (true moving-bed) model. Upward and downward gradients are compared with respect to the use of desorbent and salt.

Revue / Journal Title

AIChE journal    ISSN  0001-1541   CODEN AICEAC 

Source / Source

2003, vol. 49, no3, pp. 665-674 [10 page(s) (article)] (21 ref.)

Langue / Language

Anglais

Editeur / Publisher

Wiley, Hoboken, NJ, ETATS-UNIS  (1955) (Revue)

Mots-clés anglais / English Keywords

Experimental study

;

Numerical simulation

;

Sodium Chlorides

;

Salt effect

;

Partition coefficient

;

Breakthrough curve

;

Performance

;

Simulated moving bed

;

Ion exchange chromatography

;

Liquid chromatography

;

Preparative chromatography

;

Protein

;

Purification

;

Separation method

;

Mots-clés français / French Keywords

Etude expérimentale

;

Simulation numérique

;

Sodium Chlorure

;

Effet sel

;

Coefficient partage

;

Courbe rupture

;

Performance

;

Lit mobile simulé

;

Chromatographie échange ion

;

Chromatographie phase liquide

;

Chromatographie préparative

;

Protéine

;

Purification

;

Méthode séparation

;

Mots-clés espagnols / Spanish Keywords

Estudio experimental

;

Simulación numérica

;

Sodio Cloruro

;

Efecto sal

;

Coeficiente repartición

;

Curva ruptura

;

Rendimiento

;

Lecho móvil simulado

;

Cromatografía cambio iónico

;

Cromatografía fase líquida

;

Cromatografía preparativa

;

Proteína

;

Purificación

;

Método separación

;

Localisation / Location

INIST-CNRS, Cote INIST : 7678, 35400010942713.0100

Nº notice refdoc (ud4) : 14608198



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