Titre du document / Document title

The micromorphology and protein characterization of rubber particles in Ficus carica, Ficus benghalensis and Hevea brasiliensis

Auteur(s) / Author(s)

SINGH Adya P. ⁽¹⁾ ; SEUNG GON WI ⁽¹⁾ ; GAP CHAE CHUNG ⁽²⁾ ; YOON SOO KIM ⁽¹⁾ ; HUNSEUNG KANG ^{(2 3)} ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Department of Forest Products and Technology, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, COREE, REPUBLIQUE DE
⁽²⁾ Division of Applied Plant Science and Agricultural Plant Stress Research Center, College of Agriculture and Life Sciences, Chonnam National University, 300 Yongbong-dong, Buk-gu, Gwangju, 500-757, COREE, REPUBLIQUE DE
⁽³⁾ Kumho Life and Environmental Science Laboratory, 1 Oryong-dong, Buk-gu, Gwangju, 500-712, COREE, REPUBLIQUE DE

Résumé / Abstract

Rubber biosynthesis takes place on the surface of rubber particles. These particles are surrounded by a monolayer membrane in which the rubber transferase is anchored. In order to gain better insight into whether rubber particles from different plant species share common structural characteristics, the micromorphology of rubber particles from Ficus carica, Ficus benghalensis, and Hevea brasiliensis was examined by electron microscopy. Rubber particles of all three species were spherical in shape, and the size of rubber particles of H. brasiliensis was much smaller than those of F. carica and F. benghalensis. In addition, investigations were undertaken to compare the cross-reactivity of the antibody raised against either the H. brasiliensis small rubber particle protein (SRPP) which is suggested to be involved in rubber biosynthesis, or the cis-prenyltransferase (CPT) which has an activity similar to rubber transferase. Both western analysis and TEM-immunogold labelling studies showed that rubber particles of F. carica and F. benghalensis do not contain the SRPP. None of the rubber particles in F. carica, F. benghalensis and H. brasiliensis contained the CPT, suggesting that the CPT itself could not catalyse the formation of high molecular weight rubber. These results indicate that rubber particles in the three different plant species investigated share some degree of similarity in architecture, and that the SRPP and CPT themselves are not the core proteins necessary for rubber biosynthesis.

Revue / Journal Title

Journal of experimental botany   ISSN 0022-0957   CODEN JEBOA6 

Source / Source

2003, vol. 54, n^o384, pp. 985-992 [8 page(s) (article)] (32 ref.)

Langue / Language

Anglais

Editeur / Publisher

Oxford University Press, Oxford, ROYAUME-UNI  (1950) (Revue)

Mots-clés anglais / English Keywords

Laticiferous plant ; Fruit tree ; Euphorbiaceae ; Spermatophyta ; Angiospermae ; Dicotyledones ; Moraceae ; Enzyme ; Transferases ; Molecular structure ; Hevea brasiliensis ; Ficus carica ; Interspecific comparison ; Particle ; Rubber ; Metabolic pathway ; Biosynthesis ; Morphology ; Dimethylallyltranstransferase ; Rubber cis-polyprenylcistransferase ; Characterization ;

Mots-clés français / French Keywords

Plante à latex ; Arbre fruitier ; Euphorbiaceae ; Spermatophyta ; Angiospermae ; Dicotyledones ; Moraceae ; Enzyme ; Transferases ; Ficus benghalensis ; Structure moléculaire ; Hevea brasiliensis ; Ficus carica ; Comparaison interspécifique ; Particule ; Caoutchouc ; Voie métabolique ; Biosynthèse ; Morphologie ; Dimethylallyltranstransferase ; Rubber cis-polyprenylcistransferase ; Caractérisation ;

Mots-clés espagnols / Spanish Keywords

Planta de latex ; Arbol frutal ; Euphorbiaceae ; Spermatophyta ; Angiospermae ; Dicotyledones ; Moraceae ; Enzima ; Transferases ; Estructura molecular ; Hevea brasiliensis ; Ficus carica ; Comparación interespecífica ; Partícula ; Caucho ; Vía métabolica ; Biosíntesis ; Morfología ; Dimethylallyltranstransferase ; Rubber cis-polyprenylcistransferase ; Caracterización ;

Mots-clés d'auteur / Author Keywords

Electron microscopy ; Ficus benghalensis ; Ficus carica ; Hevea brasiliensis ; immunolocalization ; micromorphology ; rubber particle ; rubber particle protein ;

Localisation / Location

INIST-CNRS, Cote INIST : 6923, 35400010779388.0110