Titre du document / Document title

Thermogenesis inhibition in Brown adipocytes is a specific property of volatile anesthetics

Auteur(s) / Author(s)

OHLSON Kerstin B. E. ^{(1 2)} ; LINDAHL Sten G. E. ⁽³⁾ ; CANNON Barbara ⁽¹⁾ ; NEDERGAARD Jan ⁽¹⁾ ;

Affiliation(s) du ou des auteurs / Author(s) Affiliation(s)

⁽¹⁾ Wenner-Gren Institute The Arrhenius Laboratories F3, Stockholm University, Stockholm, SUEDE
⁽²⁾ Department of Anesthesiology and Intensive Care, Lund University Hospital, Lund, SUEDE
⁽³⁾ Karolinska Hospital, Stockholm, SUEDE

Résumé / Abstract

Background: This investigation examined the possibility that the inhibitory effect of halothane on nonshivering thermogenesis (heat production) in brown adipocytes is not a universal effect of all anesthetic agents but related to the type of anesthetic. Methods: Brown adipocytes from hamster were Lsolated with a collagenase digestion method and incubated with anesthetic agents. The rate of oxygen consumption was measured with an oxygen electrode. The effect of clinically relevant (and higher) doses of anesthetics of different classes on basal and norepinephrine-induced thermogenesis (oxygen consumption) was tested. Results: Two distinct groups of anesthetics could be distinguished: thermogenesis inhibitors and noninhibitors. Thermogenesis inhibitors include volatile anesthetics such as halothane (IC₅₀, 1.1 mM), ether (IC₅₀, 20 mM), and chloroform (IC₅₀, 2.2 mM) (nominal concentrations), but also tribromoethanol (IC₅₀, 0.6 mM), all inducing inhibition of norepinephrine-induced thermogenesis without affecting the EC₅₀ for norepinephrine. Thermogenesis noninhibitors include the nonvolatile anesthetics pentobarbital, propofol, ketamine, and urethane, the inhalation anesthetic nitrous oxide, and, notably, also the volatile nonanesthetics (nonimmobilizers) 1,2-dichlorohexafluorocyclobutane and 2,3-dichlorooctafluorobutane; none of these compounds had any effect on norepinephrine-induced thermogenesis at any concentration tested. Conclusions: There are two distinct classes of anesthetics with regard to effects on thermogenesis, thermogenesis inhibitors and thermogenesis noninhibitors. The results are important for the interpretation of studies in thermal biology in general; specifically, they indicate that conclusions concerning regulation of nonshivering thermogenesis during anesthesia depend on the type of anesthetic used. Of clinical importance is that the volatile anesthetics are inhibitory for nonshivering thermogenesis and thus for an alternative heat production when myorelaxants prevent shivering. As the distinction between thermogenesis inhibitors and thermogenesis noninhibitors corresponds to the distinction between volatile and nonvolatile anesthetics, it may be related to the mode of action of the volatile anesthetics.

Revue / Journal Title

Anesthesiology   ISSN 0003-3022   CODEN ANESAV 

Source / Source

2003, vol. 98, n^o2, pp. 437-448 [12 page(s) (article)] (60 ref.)

Langue / Language

Anglais

Editeur / Publisher

Lippincott, Hagerstown, MD, ETATS-UNIS  (1940) (Revue)

Mots-clés anglais / English Keywords

Vertebrata ; Mammalia ; Rodentia ; In vitro ; Animal ; Hamster ; Adipocyte ; Brown adipose tissue ; Thermogenesis ; General anesthetic ;

Mots-clés français / French Keywords

Vertebrata ; Mammalia ; Rodentia ; In vitro ; Animal ; Hamster ; Adipocyte ; Tissu adipeux brun ; Thermogenèse ; Anesthésique général ;

Mots-clés espagnols / Spanish Keywords

Vertebrata ; Mammalia ; Rodentia ; In vitro ; Animal ; Hamster ; Adipocito ; Tejido adiposo cafe ; Termogénesis ; Anestésico general ;

Localisation / Location

INIST-CNRS, Cote INIST : 3042, 35400010393065.0230